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Purified anti-mouse Podocalyxin Antibody

Pricing & Availability
Clone
10B9 (See other available formats)
Regulatory Status
RUO
Other Names
Podocalyxin
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
10B9_PURE_Podocalyxin_Antibody_112221
Mouse endothelial cell line, bEnd.3, was stained with purified anti-mouse Podocalyxin (filled histogram) or rat IgG1, κ isotype control (open histogram) followed by anti-rat IgG PE.
  • 10B9_PURE_Podocalyxin_Antibody_112221
    Mouse endothelial cell line, bEnd.3, was stained with purified anti-mouse Podocalyxin (filled histogram) or rat IgG1, κ isotype control (open histogram) followed by anti-rat IgG PE.
  • 10B9_PURE_Podocalyxin_Antibody_2_112221
    C57BL/6 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. The section was stained with 5 µg/mL purified anti-mouse Podocalyxin followed by anti-mouse IgG Alexa Fluor® 555. Nuclei were counterstained with DAPI (blue). The image was captured by a 20X objective.
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107902 100 µg £217
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Description

Podocalyxin is sialomucin, most closely related to CD34 and endoglycan, expressed by kidney podocytes, hematopoietic progenitors, vascular endothelia, and a subset of neurons. It regulates both cell adhesion and cell morphology and plays an essential role in the formation of podocyte foot processes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
OSM-dependent endothelial-like cell line, LO
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Onitsuka, I., et al., 2010. Gastroenterology, 138:1525-1535.
  2. Doyonnas, R., et al., 2005. Blood, 105:4170-4178.
  3. Minegishi, N., et al., 2003. Blood, 102:896-905.
  4. Minehata, K., et al., 2002. Blood, 99:2360-2368.
  5. Doyonnas, R., et al., 2001. J. Exp. Med., 194:13-28.
  6. Hara, T., et al., 1999. Immunity, 11:567-578.
RRID
AB_2904281 (BioLegend Cat. No. 107902)

Antigen Details

Structure
Single-pass type I membrane protein, sialoglycoprotein
Distribution

Apical cell membrane, membrane raft

Function
Cell adhesion, cell migration
Interaction
Forms a complex with EZR and SLC9A3R2.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Immunology
Antigen References
  1. Refaeli I, et al. 2020. Scientific Reports 10:9419
  2. Economou C, et al. 2004. J Cell Sci (2004) 117 (15): 3281–94.
  3. Cait J, et al. 2019. Proc Natl Acad Sci. 116 (10) 4518-4527
  4. Doyonnas R, et al. 2005. Blood. 105(11):4170-8
  5. Zhang H, et al. 2014. Stem Cells.  32(1):191-203
Gene ID
27205 View all products for this Gene ID
UniProt
View information about Podocalyxin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/22/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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