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Purified anti-mouse/rat CD62P (P-selectin) Antibody

Pricing & Availability
Clone
RMP-1 (See other available formats)
Regulatory Status
RUO
Other Names
GMP-140, Platelet activation-dependent granule membrane protein (PADGEM)
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
RMP-1_Purified_CD62P_Antibody_FC_072814.jpg
Thrombin stimulated C57BL/6 mouse platelets were stained with purified CD62P (clone RMP-1) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • RMP-1_Purified_CD62P_Antibody_FC_072814.jpg
    Thrombin stimulated C57BL/6 mouse platelets were stained with purified CD62P (clone RMP-1) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.
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148301 50 µg £73
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148302 500 µg £213
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Description

CD62P is a 140 kD type I transmembrane glycoprotein, also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62P is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells, and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. A primary function of CD62P is cell adhesion during neutrophil rolling, and platelet-neutrophil and platelet-monocyte interactions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Thrombin activated rat platelets
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, IHC, WB, IP, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence6, immunohistochemistry5, ELISA7, Western blotting4, immunoprecipitation4, and blocking in rats1,2 and mice3.

Application References

(PubMed link indicates BioLegend citation)
  1. Walter UM, et al. 1997. Immunology. 92:290. (Block-rats)
  2. Johnston B, et al. 1997. J. Immunol. 159:4514. (Block-rats)
  3. Hickey MJ, et al. 1998. Circ. Res. 83:1124. (Block-mice)
  4. Walter UM, et al. 1997. Hybridoma. 16:249. (WB, IP)
  5. Alvarez A, et al. 2004. Blood 104:402. (IHC)
  6. Piccio L, et al. 2002. J. Immunol. 168:1940. (IF)
  7. Walter UM, et al. 1997. Hybridoma. 16:249. (ELISA)
Product Citations
  1. Zhou M, et al. 2023. Biomed Pharmacother. 161:114530. PubMed
  2. Scherlinger M, et al. 2021. Sci Transl Med. 13:. PubMed
  3. Lorsung RM, et al. 2020. Front Bioeng Biotechnol. 0.9375. PubMed
  4. Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
  5. Namachivayam K, et al. 2020. Proc Natl Acad Sci U S A. 117:10958. PubMed
RRID
AB_2564110 (BioLegend Cat. No. 148301)
AB_2564110 (BioLegend Cat. No. 148302)

Antigen Details

Structure
Type I transmembrane glycoprotein, 140 kD
Distribution

Activated platelets, megakaryocytes, endothelial cells

Function
Adhesion, neutrophil rolling, platelet-neutrophil and platelet-monocyte interactions
Ligand/Receptor
CD162 (PSGL-1), CD24 and sialylated Lewis X
Cell Type
Endothelial cells, Megakaryocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. McEver R, et al. 1995. J. Biol. Chem. 270:11025.
2. Varki A. 1994. Proc. Natl. Acad. Sci. USA 91:7390.

Gene ID
20344 View all products for this Gene ID 25651 View all products for this Gene ID
UniProt
View information about CD62P on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01/25/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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