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Purified anti-mouse/rat/human MIF Antibody

Pricing & Availability
Clone
Poly5240 (See other available formats)
Regulatory Status
RUO
Other Names
Macrophage migration inhibitory factor (MMIF), Glycosylation inhibiting factor (GIF), GLIF, Phenylpyruvate Tautomerase, L-Dopachrome Tautomerase, L-Dopachrome Isomerase
Isotype
Goat Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly5240_PURE_MIF_Antibody_1_112818
Total cell lysates (15 µg total protein) from 293T, A431, U937 (reduced expression control), HeLa (reduced expression control), and UMR106 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1:500 diluted Purified anti-MIF Antibody, clone Poly5240, overnight at 4°C. Proteins were visualized by chemiluminescence detection using donkey anti-goat IgG Antibody at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Cell lysates were loaded in order of decreasing MIF mRNA expression levels (source: Human Protein Atlas).
  • Poly5240_PURE_MIF_Antibody_1_112818
    Total cell lysates (15 µg total protein) from 293T, A431, U937 (reduced expression control), HeLa (reduced expression control), and UMR106 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1:500 diluted Purified anti-MIF Antibody, clone Poly5240, overnight at 4°C. Proteins were visualized by chemiluminescence detection using donkey anti-goat IgG Antibody at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Cell lysates were loaded in order of decreasing MIF mRNA expression levels (source: Human Protein Atlas).
  • Poly5240_PURE_MIF_Antibody_2_112818
    Total cell lysates (15 µg total protein) from Raw 264.7 and A20 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1:1000 diluted Purified anti-MIF Antibody, clone Poly5240, overnight at 4°C. Proteins were visualized by chemiluminescence detection using donkey anti-goat IgG antibody at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
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524001 200 µL £221
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Description

MIF (human macrophage migration inhibitory factor) is a non-glycosylated polypeptide expressed by multiple cell types, which include activated T cells, macrophages, eosinophils, epithelial cells, and endothelial cells. It is secreted by response to diverse inflammatory stimuli, and has been associated with a clear pro-inflammatory and proatherogenic role in multiple studies involving patients and animals. MIF also positively correlates with obesity as well as insulin resistance and contributes to adipose tissue inflammation by modulating ATM functions. Furthermore, MIF has been described as a protein that plays an essential role in both innate and acquired immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat, Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Purified recombinant mouse MIF (AA: Pro2-Ala115) expressed in E. coli (Cat# 599504).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, store undiluted at -20°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10.0 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2562323 (BioLegend Cat. No. 524001)

Antigen Details

Structure
115 amino acids with a predicted molecular weight of approximately 12.5 kD.
Distribution

Activated T-cells, macrophages, eosinophils, epithelial cells, and endothelial cells.

Function
MIF has been associated with a clear pro-inflammatory and proatherogenic role in cardiovascular diseases. MIF also plays important roles in adipose tissue inflammation and innate and acquired immunity.
Interaction
Monocytes and macrophages, mesenchymal cells, and B-cells.
Ligand/Receptor
CD44/CD74, CXCR4, CXCR2, and CXCR7.
Cell Type
Endothelial cells, Eosinophils, Epithelial cells, Macrophages, T cells
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Zhou H, et al. 2009. PLoS One. 4:e6087.
2. Potolicchio I, et al. 2003. J. Biol. Chem. 278:30889.
3. Meyer-Siegler K and Iczkowski K. 2005. BMC Cancer 5:73.
4. Babu SN, et al. 2012. Asian Pac. J. Cancer Prev. 13:1737.
5. van der Vorst EP, et al. 2015. Front Immunol. 6:373.
6. Kim BS, et al. 2015. Exp. Mol. Med. 47:e161.
7. Sanchez-Zamora YI, et al. 2014. J. Diabetes Res. 2014:804519.
8. Gilliver SC, et al. 2011. Exp. Dermatol. 20:1.
9. Bach JP, et al. 2008. Oncology 75:127.

Gene ID
17319 View all products for this Gene ID
UniProt
View information about MIF on UniProt.org
Go To Top Version: 1    Revision Date: 12/02/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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