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Purified anti-mouse Tim-4 Antibody

Pricing & Availability
Clone
RMT4-54 (See other available formats)
Regulatory Status
RUO
Other Names
T cell immunoglobulin and mucin domain containing protein-4
Isotype
Rat IgG2a, κ
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Product Citations
publications
RMT4-54_Pure_Tim-4_Antibody_2_030518
Peyer’s patches of a CX3CR1-GFP -/+ mouse were fixed and cryosectioned in OCT. Following blocking and permeabilization of tissues slices, they were stained overnight at 4°C with the primary antibodies Rt anti-Tim4 (RMT4-54), Hm anti-CD11c (N418), Chk anti-GFP followed by 1h at RT with the secondary antibodies Dk anti-rat Cy3 (pink), Gt anti-hamster Alexa Fluor® 594 (red), and Dk anti-chicken Alexa Fluor® 488 (green/yellow). Following additional blocking, sections were then stained with anti-CD4 (RM4-5) eFluor 450 (cyan) for one hour, washed in PBS and mounted in Prolong Gold™. Merged image is on the left and single color image of anti-Tim-4 is on the right. Image Courtesy of Hugues Lelouard, Centre d’immunologie de Marseille-Luminy.
  • RMT4-54_Pure_Tim-4_Antibody_2_030518
    Peyer’s patches of a CX3CR1-GFP -/+ mouse were fixed and cryosectioned in OCT. Following blocking and permeabilization of tissues slices, they were stained overnight at 4°C with the primary antibodies Rt anti-Tim4 (RMT4-54), Hm anti-CD11c (N418), Chk anti-GFP followed by 1h at RT with the secondary antibodies Dk anti-rat Cy3 (pink), Gt anti-hamster Alexa Fluor® 594 (red), and Dk anti-chicken Alexa Fluor® 488 (green/yellow). Following additional blocking, sections were then stained with anti-CD4 (RM4-5) eFluor 450 (cyan) for one hour, washed in PBS and mounted in Prolong Gold™. Merged image is on the left and single color image of anti-Tim-4 is on the right. Image Courtesy of Hugues Lelouard, Centre d’immunologie de Marseille-Luminy.
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130002 200 µg £201
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Description

Tim-4 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed on antigen-presenting cells and not on T cells. Tim-4 binds to Tim-1 to promote T cell proliferation by enhancing cell division and reducing apoptosis. Tim-4 bind to phosphatidylserine through its FG-CC’ binding cleft in the N-terminal IgV domain to facilitate the clearance of apoptotic cells or bodies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse TIM4-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Customer reported
FA, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats of this clone) include: in vivo induction of auto-antibody production1 and blockade of dendritic cell Tim-42.

Application References

(PubMed link indicates BioLegend citation)
  1. Nakayama M, et al. 2009. Blood. 113:3821. (FA)
  2. Yeung MY, et al. 2013. J. Immunol. 191:4447. (Block)
Product Citations
  1. Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
  2. Khan T, et al. 2013. J Immunol. 190:1433. PubMed
  3. Dasgupta D, et al. 2020. Gastroenterology. 159:1487. PubMed
  4. O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed
  5. Ipseiz N, et al. 2020. EMBO J. 39:e103454. PubMed
RRID
AB_1227802 (BioLegend Cat. No. 130002)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain
Distribution

Unlike the other Tims, Tim-4 is only expressed by antigen-presenting cells, but not by T cells.

Function
Tim-4 is expressed on antigen-presenting cells. It provides co-stimulation to promote T cell proliferation and survival and has a possible role in clearance of apoptotic cells by binding phosphatidylserine.
Interaction
Phosphatiylserine is the ligand of Tim-4 and Tim-4 is a ligand of Tim-1.
Ligand/Receptor
Phosphatidylserine, TIM1
Cell Type
Antigen-presenting cells
Biology Area
Immunology
Antigen References

1. Kuchroo VK, et al. 2008. Nat. Rev. Immunol. 8:577
2. Miyanishi M, et al. 2007. Nature 450:435
3. Rodriguez-Manzanet R, et al. 2008. J. Immunol. 180:4706

Gene ID
276891 View all products for this Gene ID
UniProt
View information about Tim-4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 03/05/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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