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Purified anti-mouse TSLP Antibody

Pricing & Availability
Clone
28F12 (See other available formats)
Regulatory Status
RUO
Other Names
Thymic stromal lymphopoietin (TSLP)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
TSLP_Mouse_ELISA(Std.)_Recombinant_100913.jpg
  • TSLP_Mouse_ELISA(Std.)_Recombinant_100913.jpg
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515201 50 µg £77
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515202 500 µg £238
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Description

TSLP was initially identified in conditioned medium from a mouse thymic stromal cell line. Human and mouse TSLP are only 43% identical at the amino acid level, and TSLP, like IL-7, exhibits species-specific functions and may function differently in humans and mice. TSLP binds to a heterodimeric receptor constituted by IL-7Ra and TSLPR. TSLPR has low affinity for TSLP, but in combination with IL-7Ra generates a high affinity binding site. TSLP has been associated with the development of allergic disorders in humans, such as asthma, atopic dermatitis, and food allergies. The susceptibility to allergic diseases has been associated to basophil induction by TSLP, and Th2 cytokine production induced by TSLP in mast cells. In addition, TSLP has been associated to the development of Tregs, and blocking TSLPR leads to delayed functional maturation of thymic Tregs. TSLPR has been detected in Tregs from allergic asthmatic patients. TSLP directly activates STAT5 in Tregs and suppresses its suppressive activities; additionally, it inhibits IL-10 production. Human TSLP induces the release of T cell-attracting chemokines (CCL17 and CCL22) from monocytes and enhances the T cell stimulatory capacity of the CD11c+ subset of dendritic cells. TSLP induces proliferation of naïve T cells. In addition, it can drive Th2 differentiation via the induction of IL-4 production, and drives Th2 cytokine-mediated inflammation by acting on bone marrow-resident progenitors, which promotes basophil hematopoiesis. TSLP is upregulated in epithelial cells by IL-1α, TNFα, and Th2 cytokines.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Thymus stromal lymphopoietin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
Neut - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of TSLP recombinant protein ranging from 2000 to 31.3 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The purified 28F12 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with biotinylated 65B12 antibody (Cat. No. 521904) as the detecting antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Sokol CL, et al. 2008. Nat. Immunol. 9(3):310-8. (Neut)
  2. Mosconi I, et al. 2013. Mucosal Immunology. 6:1157. (Neut)
  3. Cahenzli J, et al. 2013. Cell Host Microbe. 14:559. (Neut)
  4. Marshall NB, et al. 2015. Toxicol.Sci. 147(1):127-39. (Neut)
Product Citations
  1. Laliberté A, et al. 2023. iScience. 108351:26. PubMed
RRID
AB_2561323 (BioLegend Cat. No. 515201)
AB_2561323 (BioLegend Cat. No. 515202)

Antigen Details

Structure
Cytokine
Distribution

Epithelial cells, mast cells, basophils, epidermal keratinocytes in atopic dermatitis, synovial fibroblast in arthritis rheumatoid, human airway smooth muscle cells

Function
Induces proliferation of naïve T cells, the release of T cell-attracting chemokines (CCL17 and CCL22) from monocytes, and the T cell stimulatory capacity of CD11c+ subset of dendritic cells; drives Th2 differentiation, drives Th2 cytokine-mediated inflammation to promote basophil hematopoiesis
Interaction
Monocyte, dendritic cells, activated human basophils, activated human T cells, Langerhans cells, B cells, mast cells, activated pulmonary Tregs, NKT cells
Ligand/Receptor
Heterodimeric receptor complex composed of the IL-7 receptor α chain (IL-7Rα) and the TSLP receptor (TSLPR or CRLF2)
Cell Type
Basophils, Epithelial cells, Fibroblasts, Mast cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Levin SD, et al. 1999. J. Immunol. 162:677.
2. Reche PA, et al. 2001. J. Immunol. 167:336.
3. Omori M and Steven Ziegler. 2007. J. Immunol. 178:1396.
4. Rochman I, et al. 2007. J. Immunol. 178:6720.
5. Mazzucchelli R, et al. 2008. Blood 112:3283.
6. Siracusa MC, et al. 2011. Nature 477:229.
7. Nguyen KD, et al. 2010. Allergy Asthma Clin. Immunol. 6:4.

Gene ID
53603 View all products for this Gene ID
UniProt
View information about TSLP on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/06/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Purified anti-mouse TSLP

    TSLP_Mouse_ELISA(Std.)_Recombinant_100913.jpg
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