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Purified anti-MTHFD1 Antibody

Pricing & Availability
Clone
W22086A (See other available formats)
Regulatory Status
RUO
Other Names
C-1-tetrahydrofolate synthase, cytoplasmic; CIMAH; MTHFC; MTHFD
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. W22086A_PURE_MTHFD1_WB_102323
Whole cell extracts (15 µg total protein per lane) from the indicated cell lines (lane 2 and 3 are 293T cells without and with MTHFD1 knockdown, respectively) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with the 1 µg/mL of purified anti-MTHFD1 (clone W22086A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:5000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W22086A_PURE_MTHFD1_WB_102323
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines (lane 2 and 3 are 293T cells without and with MTHFD1 knockdown, respectively) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with the 1 µg/mL of purified anti-MTHFD1 (clone W22086A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:5000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W22086A_PURE_MTHFD1_ICC_102323
    HeLa cells without (panel A and B) and with (panel C and D) MTHFD1 knockdown were fixed with ice-cold methanol, and then blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with 2 µg/mL of purified anti-MTHFD1 (clone W22086A), followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 Goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The image was captured with a 40X objective. Scale bar: 25 µm
Cat # Size Price Quantity Check Availability Save
629951 25 µg £113
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629952 100 µg £281
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Description

MTHFD1 is a trifunctional protein containing 10-formyltetrahydrofolate synthetase, 5,10-methenyltetrahydrofolate cyclohydrolase and 5,10-methylenetetrahydrofolate dehydrogenase activities and involved in the tetrahydrofolate interconversion pathway, which is part of one-carbon (1C) metabolism. MTHFD1 consists of two major domains, an N-terminal containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain in the C-terminus. According to reports, fibroblasts of patients with MTHFD deficiency exhibited enriched MTHFD1 in the nucleus, leading to low rate of de novo dTMP synthesis. Mutations in the MTHFD1 gene in pregnant women are associated with increased risk of giving birth to children with neural tube defects, along with possible decreased fetal survival. As folate metabolism is involved in migraine pathophysiology, particularly migraine with aura, MTHFD1 is also involved in migraine pathogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial Recombinant human MTHFD1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verifiied
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. 

Application Notes

The clone cross-reacts with mouse in western blot (WB).

For immunocytochemistry (ICC), ice-cold methanol fixation is recommended. 

This product is not recommended for use in immunohistochemistry (IHC).

Additional Product Notes

This antibody has been tested in knockout/knockdown models for Western Blotting and Immunocytochemistry.

RRID
AB_3097335 (BioLegend Cat. No. 629951)
AB_3097335 (BioLegend Cat. No. 629952)

Antigen Details

Structure
MTHFD1 protein is a 935 amino acid protein with a predicted molecular weight of 102 kD
Distribution

Ubiquitous

Function
Nucleotide synthesis
Cell Type
Fibroblasts
Biology Area
Cell Biology
Molecular Family
Enzymes and Regulators
Antigen References
  1. Krajinovic M. 2008. PHARMACOGENOMICS. 9:829-32.
  2. Field MS. et al. 2014. Proc Natl Acad Sci USA. 112(2):400-5.
  3. Watkins D, et al. 2011. Journal of Medical Genetics. 48:590-2.
  4. Parle-McDermott A, et al. 2005. Mol Hum Reprod. 11:477-80.
  5. Sutherland HG, et al. 2014. Headache. 54:1506-14.
Gene ID
4522 View all products for this Gene ID
UniProt
View information about MTHFD1 on UniProt.org
Go To Top Version: 1    Revision Date: 10/24/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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