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Purified anti-Myogenin Antibody

Pricing & Availability
Clone
F5D (See other available formats)
Regulatory Status
RUO
Other Names
Myogenin (MYOG), Class C basic helix-loop-helix protein 3 (BHLHC3), Myogenic factor 4 (Myf-4)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
F5D_PURE_Myogenin_Antibody_103122
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) of purified anti-Myogenin (clone F5D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • F5D_PURE_Myogenin_Antibody_103122
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) of purified anti-Myogenin (clone F5D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
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608851 25 µg £107
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608852 100 µg £238
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Description

Myogenin is a basic helix-loop-helix transcription factor from the myogenic regulator factor (MRF) family of transcription factors, which also includes MyoD, Myf5 and MRF4. Like other members of the MRF family myogenin is required for skeletal muscle development, and regulates the growth, repair and atrophy of skeletal muscle and myosatellite cells. In mice, loss of myogenin expression results in perinatal lethality resulting from major deficits in skeletal muscle formation. In humans, myogenin has diagnostic value as a marker for rhabdomyosarcoma and other related cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide containing a fragment of the N-terminus of rat myogenin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product was tested for use in immunocytochemistry and flow cytometry in HeLa and RD cells but did not meet passing criteria.

This product was tested for use in IHC-P in human and mouse skeletal muscle tissue but did not meet passing criteria.

RRID
AB_2927974 (BioLegend Cat. No. 608851)
AB_2927974 (BioLegend Cat. No. 608852)

Antigen Details

Structure
Myogenin is a 224 amino-acid protein with a predicted molecular weight of 25.0 kd.
Distribution

Myogenin is expressed in the nucleus of skeletal muscle and myosatellite cells.

Function
Transcription factor
Interaction
Myogenin interacts with MDFI, POLR2C, SRF, SP1, and TCF3
Biology Area
Cell Biology, Transcription Factors
Antigen References
  1. Boyer J, et al. 2014. Hum Mol Genet. 23:4249-59.
  2. Nabeshima Y, et al. 1993. Nature. 346:532-5.
  3. Zammit P. 2017. Semin Cell Dev Biol. 72:19-32
Gene ID
4656 View all products for this Gene ID
UniProt
View information about Myogenin on UniProt.org
Go To Top Version: 1    Revision Date: 10/31/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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