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Purified anti-Myosin heavy chain 11 Antibody

Pricing & Availability
Clone
SM-M5 (See other available formats)
Regulatory Status
RUO
Other Names
MYH11, smooth muscle Myosin heavy chain 11, SMMHC
Isotype
Mouse IgG1, κ
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Product Citations
publications
A. SM-M5_PURE_-Myosin-heavy-chain-11_IHC-P_101624
HC staining with Purified anti-Myosin heavy chain 11 (clone SM-M5) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated either without (panel A) or with (panel B) Purified anti-Myosin heavy chain 11 (clone SM-M5) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • A. SM-M5_PURE_-Myosin-heavy-chain-11_IHC-P_101624
    HC staining with Purified anti-Myosin heavy chain 11 (clone SM-M5) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated either without (panel A) or with (panel B) Purified anti-Myosin heavy chain 11 (clone SM-M5) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • b. SM-M5_PURE_-Myosin-heavy-chain-11_WB1_101624
    Whole cell extracts (15 µg total protein per lane) from indicated human cell lines and/or tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti- Myosin heavy chain 11 (clone SM-M5) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • c. SM-M5_PURE_-Myosin-heavy-chain-11_WB2_101624
    Whole cell extracts (15 µg total protein per lane) from indicated mouse cell lines and/or tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti- Myosin heavy chain 11 (clone SM-M5) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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673351 25 µg £100
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673352 100 µg £278
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Description

Myosin Heavy Chain 11, also known as MYH11, is a key protein involved in the contractile function of smooth muscle cells. This protein is a major component of the myosin II complex, which interacts with actin filaments to facilitate muscle contraction. By utilizing ATP, MYH11 enables the sliding of actin filaments, generating the mechanical force necessary for smooth muscle contraction. This function is crucial for maintaining the tone and proper functioning of various smooth muscle tissues, including those in the blood vessels, gastrointestinal tract, and respiratory system. In addition to its role in muscle contraction, MYH11 is also important for the structural integrity of smooth muscle cells. Mutations in the MYH11 gene can lead to serious medical conditions, such as familial thoracic aortic aneurysms and dissections (TAAD), where the weakening of the aortic wall poses a significant risk. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Crude human uterus lysate preparation
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

For Western blot (WB), this antibody has significantly higher signal when detecting mouse Myosin heavy chain 11, as compared to human. 

This antibody is not recommended for use in immunocytochemistry (ICC), or intracellular flow cytometry (ICFC) due to dim signal.

Antigen Details

Structure
Myosin heavy chain 11 is a 1,972 amino acid protein with a predicted molecular weight of 227 kD.
Distribution

Cytoskeletal, cytoplasmic

Function
Cytoskeleton, contractile protein via ATP hydrolysis
Interaction
Myosin is a hexameric protein consisting of 2 heavy chains, 2 light chains, and 2 regulatory light chain subunits.
Biology Area
Cardiovascular Biology, Cell Motility/Cytoskeleton/Structure
Antigen References
  1. Touyz RM, et al. 2018. Cardiovasc Res. 114:529-39. 
  2. Babu GJ, et al. 2000. Microsc Res Tech. 50:532-40. 
  3. Finsterer J. 2024. Forensic Sci Med Pathol. 20:756-7.
Gene ID
4629 View all products for this Gene ID
UniProt
View information about Myosin heavy chain 11 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10/22/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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