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Purified anti-NALCN Antibody

Pricing & Availability
Clone
N185/7 (See other available formats)
Regulatory Status
RUO
Other Names
Sodium leak channel non-selective protein, Four domain-type voltage-gated ion channel alpha-1 subunit, Voltage-gated channel-like protein 1, Vgcnl1, Nca and Cankon
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
N185-7_Purified_NALCN_Antibody_112018
Western blot of purified anti-NALCN antibody (clone N185/7). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain membrane lysate; Lane 3: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N185-7_Purified_NALCN_Antibody_112018
    Western blot of purified anti-NALCN antibody (clone N185/7). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain membrane lysate; Lane 3: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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857201 25 µg £57
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857202 100 µg £113
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Description

NALCN is a voltage independent and non-selective ion channel highly expressed in the central nervous system, and permeable to Na+, K+, and Ca2+. Knockout of mouse NALCN gene results in neonatal lethality within 24 hours of post birth due to defects in respiratory rhythm. In hippocampal neurons, the activation of NALCN is regulated by neuropeptides such as neurotensin and Substance P (SP). The binding of SP to its receptor, Substance P receptor (SPR), activates the Src family of tyrosine kinases (SFK) and promotes its binding to mUNC80, a scaffold protein that recruits SFK to NALCN.

NALCN is also expressed in pancreatic islet cells where its function is regulated by the acetylcholine and M3 muscarinic receptor (M3R).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein corresponding to amino acids 1659-1738 (cytoplasmic C-terminus) of rat NALCN
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783469 (BioLegend Cat. No. 857201)
AB_2783469 (BioLegend Cat. No. 857202)

Antigen Details

Structure
Rodent NALCN is a 1,738 amino acid protein with a molecular mass of 200 kD.
Distribution

Tissue distribution: Brain, spinal cord, heart, pituitary and adrenal glands, and pancreatic islet cells.
Cellular distribution: Plasma membrane.

Function
NALCN participates in facilitating intracellular transport of sodium, modulation of neuronal excitability, and maintenance of respiratory rhythm.
Interaction
Substance P (SP), Substance P receptor (SPR), Src family of tyrosine kinases (SFK), mUNC80, M3 muscarinic receptor (M3R)
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules
Antigen References
  1. Lu B, et al. 2007. Cell. 129(2):371-83.
  2. Lu B, et al. 2010. Neuron. 68(3):488-99. 
  3. Swayne LA, et al. 2009. EMBO Rep. 10: 873–880.
Gene ID
338370 View all products for this Gene ID 266760 View all products for this Gene ID
UniProt
View information about NALCN on UniProt.org
Go To Top Version: 1    Revision Date: 12/11/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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