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Purified anti-Nanog Antibody

Pricing & Availability
Clone
23D2-3C6 (See other available formats)
Regulatory Status
RUO
Other Names
Homeobox Transcription Factor Nanog, Nanog Homeobox, Homeobox Protein NANOG, Homeobox Transcription Factor Nanog-Delta 48
Isotype
Mouse IgG1, κ
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Product Citations
publications
23D2-3C6_Purified_Nanog_Antibody_1_WB_051419
Total cell lysate from NTERA-2 cells (lane 1, 15 µg), NF-1 cells (lane 2, 15 µg) and HeLa (lane 3, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-Nanog antibody (clone 23D2-3C6). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • 23D2-3C6_Purified_Nanog_Antibody_1_WB_051419
    Total cell lysate from NTERA-2 cells (lane 1, 15 µg), NF-1 cells (lane 2, 15 µg) and HeLa (lane 3, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-Nanog antibody (clone 23D2-3C6). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • 23D2-3C6_Purified_Nanog_Antibody_2_ICFC_051419
    Human malignant pluripotent embryonal carcinoma cell line, NTERA-2, was treated with the Nuclear Factor Fixation and Permeabilization Buffer Set, intracellularly stained with purified anti-human Nanog (clone 23D2-3C6, filled histogram) or purified mouse IgG1, κ isotype control (open histogram), and then was followed by anti-mouse IgG PE.
  • 23D2-3C6_Purified_Nanog_Antibody_3_ICC_051419
    NTERA-2 cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 5 minutes, and then blocked with 5% FBS for 30 minutes. The cells were then intracellularly stained with 5 µg/ml anti-Nanog (clone 23D2-3C6) in a blocking buffer overnight at 4°C and followed by DyLight™ 594 (red) conjugated goat anti-mouse IgG for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The imag
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674001 25 µg £101
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674002 100 µg £213
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Description

Nanog is a transcription factor that plays key roles in the maintenance of pluripotency and self-renewal in embryonic stem cells. Nanog expression is regulated by other transcription factors, which include Sox2 and Oct-4. It also plays a role in epithelial malignancy and is enriched in cancer stem cells. The ablation of nanog is sufficient to reduce the cancer stem cell pool.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human recombinant protein expressed in bacteria
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted at 4°C
Application

WB - Quality tested
ICFC, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For flow cytometric staining, the suggested use of this reagent is ≤ 0.175 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2564570 (BioLegend Cat. No. 674001)
AB_2564570 (BioLegend Cat. No. 674002)

Antigen Details

Structure
305 amino acids with a predicted molecular weight of approximately 35 kD.
Distribution

Nuclear and cytoplasmic. Expressed by embryonic stem (ES) cells or cancer stem cells. Expression decreases with ES differentiation.

Function
Transcription regulator that plays key roles in the maintenance of pluripotency and self-renewal in embryonic stem cells.
Interaction
Interacts with SMAD1, SALL4, and ZNF281/ZFP281.
Cell Type
Embryonic Stem Cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Signal Transduction, Stem Cells, Transcription Factors
Antigen References

1. Hwang CC, et al. 2014. PLoS One 9:e105676.
2. Torres-Padilla ME, et al. 2014. Development 141:2173.
3. Iv Santaliz-Ruiz LE, et al. 2014. Int. J. Cancer 12:2741.
4. Wang ML, et al. 2013. Onco. Targets Ther. 6:1207.
5. Kim J, et al. 2008. Cell 132:1049.
6. Takahashi K, et al. 2007. Nat. Protoc. 2:3081.
7. Yu J, et al. 2007. Science 318:1917.

Gene ID
79923 View all products for this Gene ID
UniProt
View information about Nanog on UniProt.org
Go To Top Version: 2    Revision Date: 07/26/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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