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Purified anti-Nestin Antibody

Pricing & Availability
Clone
Poly22A17 (See other available formats)
Regulatory Status
RUO
Other Names
Nes
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
A_Poly22A17_PURE_Nestin_Antibody_ICC_112023
U-251 MG cells (positive cell line, panel A) and HeLa cells (low expressing cell line, panel B) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 1:50 dilution of Purified anti-Nestin (clone Poly22A17), followed by incubation with Alexa Fluor® 647 Donkey anti-rabbit IgG (Cat. No. 406414) for 1 hour at room temperature. Cells were also stained with Flash Phalloidin™ Green 488 (Cat. No. 424201) and nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured by a Revvity Operetta CLS™ High Content Analysis System with a 60X objective. Scale bar: 25 μm
  • A_Poly22A17_PURE_Nestin_Antibody_ICC_112023
    U-251 MG cells (positive cell line, panel A) and HeLa cells (low expressing cell line, panel B) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 1:50 dilution of Purified anti-Nestin (clone Poly22A17), followed by incubation with Alexa Fluor® 647 Donkey anti-rabbit IgG (Cat. No. 406414) for 1 hour at room temperature. Cells were also stained with Flash Phalloidin™ Green 488 (Cat. No. 424201) and nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured by a Revvity Operetta CLS™ High Content Analysis System with a 60X objective. Scale bar: 25 μm
  • B_Poly22A17_PURE_Nestin_Antibody_WB_112023
    Whole cell extracts (15 μg total protein) from indicated cell lines were resolved by 3-8% Tris-Acetate gel electrophoresis, transferred to a PVDF membrane, and probed with 1:10000 dilution of Purified anti-Nestin (clone Poly22A17) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP Donkey anti-rabbit IgG (Cat. No. 406401). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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626351 50 µL £209
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626352 200 µL £496
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Description

Nestin is a class VI intermediate filament protein originally found in neuronal stem cells, and is required for survival and renewal of neural progenitor cells. In mixtures, Nestin forms heterodimers with vimentin or internexin, participating in the remodeling of the cell. Nestin was found to be expressed on vascular endothelial progenitor cells and various neoplasms. It is proposed to be a novel angiogenesis marker and cancer therapeutic target.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant fragment of human nestin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICC - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a dilution range of 1:50 - 1:1000 is recommended. For western blotting, the suggested dilution range of this reagent is 1:5000 - 1:50000. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For Immunocytochemistry, either 4% paraformaldehyde/Triton X-100, 4% paraformaldehyde/methanol, or methanol only is recommended for fixation/permeabilization.

RRID
AB_3097319 (BioLegend Cat. No. 626351)
AB_3097319 (BioLegend Cat. No. 626352)

Antigen Details

Structure
1621 amino acids with predicted molecular weight of 177 kD; the observed molecular weight is larger than 250 kD
Distribution

Cytosol/Neural stem cells and various cancer cells

Function
Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation
Interaction
Vimentin, alpha-internexin and FHOD3
Cell Type
Neural Stem Cells
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Antigen References
  1. Matsuda Y, et al. 2013. World J Gastroenterol. 19:42-8.
  2. Matsuda Y, et al. 2012. Med Mol Morphol. 45:59-65.
  3. Krupkova O Jr, et al. 2010. Neoplasma. 57:291-8.
  4. Hombach-Klonisch S, et al. 2008. Arch Immunol Ther Exp (Warsz). 56:165-80.
  5. Pilkington GJ. 2005. Cell Prolif. 38:423-33.
Gene ID
10763 View all products for this Gene ID
UniProt
View information about Nestin on UniProt.org
Go To Top Version: 1    Revision Date: 11/17/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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