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Purified anti-Neu5Gc Antibody

Pricing & Availability
Clone
Poly21469 (See other available formats)
Regulatory Status
RUO
Other Names
N-Glycolylneuraminic acid
Isotype
Chicken IgY
Ave. Rating
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Product Citations
publications
Poly21469_Purified_Neu5Gc_Antibody_FC_1_110813
C57BL/6 mouse splenoctyes (top) or human peripheral blood lymphocytes (bottom) were incubated with Neu5Gc Assay Blocking Solution, and then stained with chicken anti-Neu5Gc (clone Poly21469) (filled histogram) or purified chicken IgY isotype control (open histogram), followed by anti-chicken IgY FITC.
  • Poly21469_Purified_Neu5Gc_Antibody_FC_1_110813
    C57BL/6 mouse splenoctyes (top) or human peripheral blood lymphocytes (bottom) were incubated with Neu5Gc Assay Blocking Solution, and then stained with chicken anti-Neu5Gc (clone Poly21469) (filled histogram) or purified chicken IgY isotype control (open histogram), followed by anti-chicken IgY FITC.
  • Poly21469_Purified_Neu5Gc_Antibody_FC_2_110813
Cat # Size Price Quantity Check Availability Save
146903 25 µL £325
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Description

Neu5Gc (N-Glycolylneuraminic acid) is a sialic acid molecule found in most mammals but not in humans.  It is derived from the most commonly expressed sialic acid, Neu5Ac, differing from it by only one oxygen atom.  The gene CMAH that encodes for CMP-N-acetylneuraminic acid hydroxylase is responsible for the conversion of Neu5Ac to Neu5Gc.  In humans, this gene is inactive due to a deletion mutation.  As such, the human immune system sees Neu5Gc as a foreign, immunogenic molecule. Neu5Gc enters the human body primarily through dietary intake of red meat and dairy products. Neu5Gc is also incorporated, albeit to a lesser degree, into certain biopharmaceuticals, through the use of animal-derived reagents and cell lines during the manufacturing of these products. Due to its immunogenic properties, the intake and incorporation of Neu5Gc in humans causes the formation of circulating antibodies against this non-human molecule, which can lead to chronic inflammation, the exacerbation of certain diseases, and possibly reduced the efficacy of biopharmaceuticals.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Reported Reactivity
All mammals except human
Antibody Type
Polyclonal
Host Species
Chicken
Formulation
This Neu5Gc antibody is lyophilized.
Preparation
Reconstitute the contents of the antibody vial by adding 25 µl of distilled water.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
For long-term storage, store the lyophilized antibody at -20°C or -70°C. After reconstitution, store the antibody between 2°C and 8°C for up to 2 weeks. For longer term storage up to 6 months, store the reconstituted product in aliquots at -20°C.
Application

FC - Quality tested
WB, ELISA, IHC - Reported in the literature, not verified in house
Recommended Usage

Dilution ranges vary depending on the application. See our protocol sheet for full details. For flow cytometry, use 1:200 to 1:1000; for ELISA, use 1:1000 to 1:10,000; and for Western blot, use 1:1000 to 1:10,000.  For the appropriate isotype control, we recommend using Chicken IgY isotype control (see Cat. No. 146901).

Application Notes

Use caution to avoid animal-derived materials or reagents that might contain Neu5Gc, as these will interfere with anti-Neu5Gc applications.  If you require a blocking solution that does not contain Neu5Gc, we recommend purchasing Cat. No. 146901, Anti-Neu5Gc Antibody Kit, which contains anti-NeuGc antibody, chicken IgY isotype control, and Neu5Gc assay blocking solution.

Application References

(PubMed link indicates BioLegend citation)
  1. Diaz SL. et al. 2009. PLoS ONE 4:e4241. (FC, WB, ELISA, IHC)
  2. Padler-Karavani V. et al. 2008. Glycobiology 18:818.
Product Citations
  1. Lopez-Gordo E, et al. 2022. Front Med (Lausanne). 8:732095. PubMed
  2. Paul A, et al. 2020. Methods Mol Biol. 2110:59. PubMed
  3. Dagur RS, et al. 2019. BMC Immunol. 20:2. PubMed
  4. Broszeit F, et al. 2019. Cell Rep. 27:3284. PubMed
  5. Wu G, et al. 2022. Wellcome Open Res. 6:134. PubMed
  6. Sénage T, et al. 2022. Nat Med. 28:283. PubMed
  7. Tanihara F, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID
AB_2562884 (BioLegend Cat. No. 146903)

Antigen Details

Structure
Sialic acid
Distribution

Most mammals

Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Protein Synthesis
Antigen References

1. Ludwig TE, et al. 2006. Nat. Methods. 3:637.
2. Nguyen DH, 2005. J. Immunol. 175:228.
3. Bardor M, et al. 2005. 280:4228.
4. Diaz SL, et al. 2009. PLoS One 4:e4241.

Gene ID
NA
UniProt
View information about Neu5Gc on UniProt.org

Related FAQs

Why does your Anti-Neu5Gc kit (cat# 146901) come with its own blocking solution? Can I use my own?
Many common blocking solutions contain reagents such as FBS that would contaminate your samples with Neu5Gc, thus leading to generation of background. The blocking solution in our kit is guaranteed to be free of any of these contaminants.

Other Formats

View All Neu5Gc Reagents Request Custom Conjugation
Description Clone Applications
Anti-Neu5Gc Antibody Kit Poly21469 FC,ELISA,WB,IHC
Purified anti-Neu5Gc Poly21469 FC,ELISA,WB,IHC
Go To Top Version: 4    Revision Date: 01/11/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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