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Purified anti-NeuroD1 Antibody

Pricing & Availability
Clone
W22035C (See other available formats)
Regulatory Status
RUO
Other Names
Neurogenic Differentiation Factor 1, BETA2, BHF-1, MODY6, Neurogenic Helix-Loop-Helix Protein NEUROD, Class A Basic Helix-Loop-Helix Protein, Beta-Cell E-Box Transactivator 2, Basic Helix-Loop-Helix Transcription Factor, BHLHA3, T2D
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W22035C_PURE_NeuroD1_WB_041624
Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of Purified anti-NeuroD1 (clone W22035C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:5000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W22035C_PURE_NeuroD1_WB_041624
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of Purified anti-NeuroD1 (clone W22035C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:5000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W22035C_PURE_NeuroD1_ICC_041624
    IMR-32 cells (mock) (panels A and B) and knockdown cells (panels C and D) were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton-X, and then blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with 5 µg/mL of Purified anti-NeuroD1 (clone W22035C), followed by incubation with 2 µg/mL of Alexa Fluor® 594 anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) and merged (panels B and D). The image was captured with a 40X objective. Scar bar: 25 µm
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633451 25 µg £124
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633452 100 µg £325
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Description

Neurogenic differentiation factor 1, also known as NeuroD1, is essential in both the pancreas and nervous system development and plays an important role in the formation of the inner ear and mammalian retina.  NeuroD1 is a transcription factor that belongs to the basic helix-loop-helix (bHLH) protein family. These proteins interact with E-proteins by building heterodimers and binding to the conventional E-box sequence CANNTG. It regulates expression of the insulin gene, and the mutations of this gene affect type II diabetes mellitus in mouse models and in mortal clinical cases. Studies indicate that NeuroD1 also could be phosphorylated at S162, S259, S266, and S274 upon glucose stimulation in a Ca2+-dependent manner. In pancreatic β-cells, Erk phosphorylation increases NeuroD1 activity, but GSK-3β phosphorylation suppresses NeuroD1 in neurons. Within the adult cortex, NeuroD1 is a marker of mature excitatory pyramidal neurons within the upper-most layers of the cortex. Aberrations in NeuroD1 in the brain causes severe developmental abnormalities. Notably, the targeted mutation of NeuroD1 results in a profound loss of neurons in the cerebellum, hippocampal dentate gyrus, and the inner ear due to extensive cell death.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human NeuroD1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For immunocytochemistry (ICC), 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice-cold methanol is recommended.

This antibody (clone W22035C) does not cross-react with mouse and rat in western blotting (WB).

This antibody (clone W22035C) is not suitable for use in immunohistochemistry (IHC).

Additional Product Notes

This antibody has been tested in knockout/knockdown models for WB and ICC.

RRID
AB_3662316 (BioLegend Cat. No. 633451)
AB_3662316 (BioLegend Cat. No. 633452)

Antigen Details

Structure
NeuroD1 protein is a 356 amino acid protein with a predicted molecular weight of 40 kD.
Distribution

Cytoplasm, Nucleus

Function
Transcriptional activator, differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex, binds to the insulin gene E-box
Interaction
TCF3/E47, EP300, RREB1, ATOH8
Cell Type
Neurons
Biology Area
Cell Biology, Cell Proliferation and Viability, Neuroscience, Transcription Factors
Antigen References
  1. Chae J.H, et al. 2004. Mol Cells. 18:271-88.
  2. Schonhoff SE, et al. 2004. Endocrinology. 145:2639-44.
  3. Sharma A, et al. 1999. Mol Cell Biol. 19:704-13.
  4. Lee TY, et al. 2020. Exp Neurobiol. 29:189-206.
  5. Miyata T, et al. 1999. Genes Dev. 13:1647-52.
  6. Poulin G, et al. 1997. Mol Cell Biol.17:6673-82.
  7. Bormuth I, et al. 2013. J Neurosci.33:641-51.
  8. Malecki M. T, et al. 1999. Nature Genetics. 23:323-8.
  9. Tutukova S, et al. 2021. Front Mol Neurosci.14:662774.
Gene ID
4760 View all products for this Gene ID
UniProt
View information about NeuroD1 on UniProt.org
Go To Top Version: 1    Revision Date: 04/16/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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