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Purified anti-NF-κB p50 Antibody

Pricing & Availability
Clone
4D1 (See other available formats)
Regulatory Status
RUO
Other Names
NF-κB p50, nuclear factor kappa light chain enhancer in B cells p50, NF-κB1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_4D1_Pure_NF-kB-p50_Antibody_070918
Total cell lysates (15 µg protein) from Jurkat (Lane 1) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.025 µg/mL (1:20,000 dilution) Purified anti-NF-κB p50 Antibody, clone 4D1 (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated with HRP for anti-NF-κB p50 Antibody , or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1 (lower panel) as a loading control. Lane M: Molecular weight ladder.
  • 1_4D1_Pure_NF-kB-p50_Antibody_070918
    Total cell lysates (15 µg protein) from Jurkat (Lane 1) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.025 µg/mL (1:20,000 dilution) Purified anti-NF-κB p50 Antibody, clone 4D1 (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated with HRP for anti-NF-κB p50 Antibody , or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1 (lower panel) as a loading control. Lane M: Molecular weight ladder.
  • 2_4D1_PURE_NF-kappaBp50_Antibody_2_101520
    A549 cells were unstimulated (left panel) or treated with 20 ng/mL TNF-α (Cat. No. 717904, right panel) for 16 hours. Cells were then fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 2.0 µg/mL (1:250 dilution) of Purified anti-NF-κB Antibody, clone 4D1, overnight at 4°C, after which proteins were visualized with Alexa Fluor® 594 Goat anti-mouse IgG Antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
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616701 25 µg £70
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616702 100 µg £150
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Description

NF-κB p50 (nuclear factor kappa light chain enhancer in B cells p50, NF-κB1) is a member of the Rel/dorsal family. NF-κB is a transcription factor, originally identified as an activator of kappa light chain in B cells. NF-κB p50, together with another NF-κB subunit p65, binds to IκB inhibitor in cytoplasm as an inactive form. Phosphorylation of IκB releases NF-κB p50 and p65. Active form of NF-κB p50 and p65 then translocate to the nucleus and regulate target gene expression. Bacterial products and stimulation of a variety of receptors lead to NF-κB signal pathway activation. This 4D1 monoclonal antibody recognizes p105 (precursor of p50) and p50 (active form). 4D1 has been validated for Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
recombinant full-length human NF-κB p50
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store between 2°C and 8°C.
Application

WB - Quality tested
ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 0.5 µg per ml. For immunocytochemistry, a concentration range of 2.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence1 on the Amnis ImageStream 100 Imaging Flow Cytometer.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Application References

(PubMed link indicates BioLegend citation)
  1. Huang F, et al. 2007. J. Immunol. 179:6504. (IF) PubMed
  2. Cao YA, et al. 2008. J. Biol. Chem. 283:15309. PubMed
  3. Lin D, et al. 2012. J Biol Chem. 287:4299. PubMed.
Product Citations
  1. Lin D, et al. 2012. J Biol Chem. 287:4299. PubMed
  2. Herati RS, et al. 2021. Cell Reports Medicine. 2(5):100262. PubMed
  3. Kao C, et al. 2008. J Biol Chem. 283:15309. PubMed
  4. Boudesco C, et al. 2018. Blood. 132:510. PubMed
  5. Huang F, et al. 2007. J Immunol. 179:6504. PubMed
RRID
AB_315855 (BioLegend Cat. No. 616701)
AB_315855 (BioLegend Cat. No. 616702)

Antigen Details

Structure
Member of the Rel/dorsal family. NF-κB p50 is an active form, derived from NF-κB p105, a precursor form.
Distribution

Ubiquitously expressed

Function
A transcription factor, originally identified as an activator of kappa light chain in B cells
Interaction
Interacts with NF-κB p65 and IκB
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Baldwin AS, et al. 1996. Annu. Rev. Immunol. 14:649 (review).
2. Chen F, et al. 1999. Clin. Chem. 45:7 (review).

Regulation
NF-κB p50, together with another NF-κB subunit p65, binds to IκB inhibitor in cytoplasm as an inactive form. Phosphorylation of IκB releases NF-κB p50 and p65. Active form of NF-κB p50 and p65 then translocate to the nucleus and regulate target gene expression. Bacterial products and stimulation of a variety of receptors lead to NF-κB signal pathway activation.
Gene ID
4790 View all products for this Gene ID
UniProt
View information about NF-kappaB p50 on UniProt.org
Go To Top Version: 3    Revision Date: 10/15/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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