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Purified anti-NFIL3 Antibody

Pricing & Availability
Clone
W16121C (See other available formats)
Regulatory Status
RUO
Other Names
IL3BP1, E4 binding protein 4 (E4BP4), NF-IL3A, NFIL3A
Isotype
Rat IgG2a, κ
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Product Citations
publications
W16121C_PURE_NFIL3_Antibody_1_070317
Total lysates (15µg protein) from Jurkat (negative control), HepG2 and NIH3T3 cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:250 (2µg/ml), 1:500 (1µg/ml) and 1:1000 (0.5µg/ml) purified anti-NFIL3 antibody, clone W16121C (upper). Proteins were visualized using an HRP goat anti-rat-IgG secondary antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M is the Molecular weight ladder.
  • W16121C_PURE_NFIL3_Antibody_1_070317
    Total lysates (15µg protein) from Jurkat (negative control), HepG2 and NIH3T3 cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:250 (2µg/ml), 1:500 (1µg/ml) and 1:1000 (0.5µg/ml) purified anti-NFIL3 antibody, clone W16121C (upper). Proteins were visualized using an HRP goat anti-rat-IgG secondary antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M is the Molecular weight ladder.
  • W16121C_PURE_NFIL3_Antibody_2_070317
    Total lysates from Jurkat and HepG2 cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-NFIL3 antibody (clone W16121C) or competitor’s clone at manufacture’s recommended concentration (upper) or GAPDH (poly6314) antibody (lower). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-rat-IgG secondary antibody for NFIL3 or HRP Donkey anti-rabbit IgG Antibody for GAPDH and competitor’s antibody.
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698101 25 µg £70
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698102 100 µg £174
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Description

NFIL3, also known as E4BP4, belongs to the basic leucine zipper (bZIP) family of transcription factors. NFIL3 has been shown to bind to the promoter region of the IL-3 gene; IL-3 treatment induces the expression of NFIL3. NFIL3 regulates several immunological processes including Th2 and Th17 cytokine production, Th17 cell differentiation, conventional natural killer (NK) cell differentiation, development of non-NK ILC lineages, and the response of macrophages and dendritic cells. NFIL3 has been found to prevent apoptosis of pro-B lymphocytes and is involved in IgE class switching. NFIL3-deficient mice showed impaired development of NK cells and reduced expression of transcription factors ID2, EOMES, GATA3, and TBX21.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human NFIL3 recombinant protein (1-250 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2 µg/mL (1:250-1:1000 diluted). It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2687267 (BioLegend Cat. No. 698101)
AB_2687267 (BioLegend Cat. No. 698102)

Antigen Details

Structure
462 amino acids with a predicted molecular weight of 50.9 kD. Contains a basic-leucine zipper (bZIP) domain responsible for DNA binding.
Distribution

Nucleus

Function
NFIL3 is a transcription factor that regulates a variety of cellular processes in lymphocyte subsets including cytokine production, class switching, and cell differentiation.
Interaction
NFIL3 forms a homodimer and interacts with DR1, PER2, and CRY2.
Antigen References

1. Xu W, et al. 2015. Cell Rep. 10:2043.
2. Zhu C, et al. 2015. Nat. Commun. 6:6072.
3. Seillet C, et al. 2014. J. Immunol. 192:2667.
4. Keniry M, et al. 2013. Genes Dev. 27:916.
5. Kashiwada M, et al. 2011. EMBO J. 30:2071.
6. Kobayashi T, et al. 2011. J. Immunol. 186:4649.

Gene ID
4783 View all products for this Gene ID
UniProt
View information about NFIL3 on UniProt.org

Related FAQs

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Go To Top Version: 0    Revision Date: 07/05/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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