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Purified anti-NG2 Antibody

Pricing & Availability
Clone
MEL62 (See other available formats)
Regulatory Status
RUO
Other Names
CSPG4, Chondroitin Sulfate Proteoglycan 4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MEL62_PURE_NG2_Antibody_070919.png
Western blot of purified anti-NG2 antibody (clone MEL62). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain lysate; Lane 3: 40 µg of mouse brain lysate; Lane 4: 40 µg of rat brain lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. Mouse and rat brain lysates were included as negative control to demonstrate the specificity of this antibody to human NG2.
  • MEL62_PURE_NG2_Antibody_070919.png
    Western blot of purified anti-NG2 antibody (clone MEL62). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain lysate; Lane 3: 40 µg of mouse brain lysate; Lane 4: 40 µg of rat brain lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. Mouse and rat brain lysates were included as negative control to demonstrate the specificity of this antibody to human NG2.
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867701 25 µg £73
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867702 100 µg £189
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Description

CSPG4, best known as neuron-glial antigen 2 (NG2), is a chondroitin sulfate proteoglycan that is frequently used as a marker for oligodendrocyte progenitor cells. NG2 plays an important role in cell proliferation and migration. It also stabilizes cell-substratum interactions during early events in melanoma cell invasion on the endothelial basement membranes. Missense mutations in NG2 are segregated in familial forms of schizophrenia, implicating its role in neurodevelopmental disorders.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814616 (BioLegend Cat. No. 867701)
AB_2814616 (BioLegend Cat. No. 867702)

Antigen Details

Structure
CSPG4 is a 2322 amino acid protein with predicted molecular mass of ~ 250 kD and observed molecular mass of ~ 260-280 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Extracellular, plasma membrane, lysosome, and Golgi apparatus

Function
NG2 is involved in cell proliferation and migration, and plays an important role in stabilizing cell-substratum interactions.
Interaction
MPDZ, PRKCA, TNC, Laminin-1, COL5A1, COL6A2
Cell Type
Oligodendrocytes
Biology Area
Cancer Biomarkers, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Cell Structure, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Schäfer MKE and Tegeder I. 2018. Matrix Biol. 68-69:571.
  2. Birey F, et al. 2017. Curr Opin Neurobiol. 47:93.
  3. Song FE, et al. 2017. CNS Neurosci Ther. 23(7):547.
Gene ID
1464 View all products for this Gene ID
UniProt
View information about NG2 on UniProt.org
Go To Top Version: 1    Revision Date: 07/09/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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