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Purified anti-NGAL (Lipocalin-2) Antibody

Pricing & Availability
Clone
M0410B2 (See other available formats)
Regulatory Status
RUO
Other Names
Lipocalin 2, Oncogene 24p3, Neutrophil Gelatinase-Associated Lipocalin, Siderocalin LCN2, 25 kD Alpha-2-Microglobulin-Related Subunit Of MMP-9, p25, MSFI, Migration-Stimulating Factor Inhibitor, HNL
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
M0410B2_NGAL_Purified_Antibody_WB_061518
Cell lysate from A431 human cell line (lane 1), NIH3T3 mouse cell line (lane 2), mouse pancreas (lane 3) and mouse bone marrow (lane 4) (15 µg protein for each) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.125 - 1 µg/mL of Purified anti-NGAL (Lipocalin-2) Antibody (clone M0410B2) (upper). Proteins were visualized using chemiluminescence detection after incubation with a 1:3000 dilution of HRP conjugated anti-rat-IgG secondary antibody for the anti-NGAL (upper panel) antibody or a 1:5000 dilution of Direct-Blot™ HRP anti-β-Actin (clone 2F1-1, lower panel). Lane M: Molecular weight ladder.
  • M0410B2_NGAL_Purified_Antibody_WB_061518
    Cell lysate from A431 human cell line (lane 1), NIH3T3 mouse cell line (lane 2), mouse pancreas (lane 3) and mouse bone marrow (lane 4) (15 µg protein for each) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.125 - 1 µg/mL of Purified anti-NGAL (Lipocalin-2) Antibody (clone M0410B2) (upper). Proteins were visualized using chemiluminescence detection after incubation with a 1:3000 dilution of HRP conjugated anti-rat-IgG secondary antibody for the anti-NGAL (upper panel) antibody or a 1:5000 dilution of Direct-Blot™ HRP anti-β-Actin (clone 2F1-1, lower panel). Lane M: Molecular weight ladder.
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661502 500 µg £238
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Description

NGAL (lipocalin-2) is a lipocalin family protein that plays important roles in innate immunity, inflammation, and atherosclerosis. Mouse NGAL is detected in lung, spleen, uterus, vagina, and epididymis. It is a critical component of innate immunity because of its ability to bind iron-siderophore complexes and limit bacterial growth. It is also produced by injured epithelia and is one of the most promising new markers for renal epithelial injury and cardiovascular disease (CVD).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse NGAL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Yamaguchi N, et al. 2023. IBRO Neurosci Rep. 14:253. PubMed
RRID
AB_2563987 (BioLegend Cat. No. 661502)

Antigen Details

Structure
195 amino acid monomer with a predicted molecular weight of approximately 23 kD.
Distribution

Secreted by epithelial cells, macrophages, neutrophils; increased levels have been observed in plasma, serum, and urine in various conditions, such as metastatic breast and colorectal cancer, acute kidney injury, pancreatitis and preeclampsia.

Function
Apoptosis, innate immunity, renal development.
Interaction
Disulfide-linked with MMP9.
Ligand/Receptor
SLC22A17 (24p3R).
Cell Type
Epithelial cells, Macrophages, Neutrophils
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Iqbal N, et al. 2013. Expert Opin. Med Diagn. 7:209.
2. Singer E, et al. 2013. Acta. Physiol. (Oxf) 207:663.
3. Palazzuoli A, et al. 2012. Clin. Chim. Acta. 413:1832.
4. Flo TH, et al. 2004. Nature 432:917.
5. Goetz DH, et al. 2002. Mol. Cell. 10:1033.
6. Leung L, et al. 2012. PLoS One 7:e46677.
7. Kjeldsen L, et al. 1993. J. Biol. Chem. 268:10425.

Gene ID
3934 View all products for this Gene ID
UniProt
View information about NGAL on UniProt.org
Go To Top Version: 2    Revision Date: 03/11/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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