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Purified anti-NGAL (Lipocalin-2) Antibody

Pricing & Availability
Clone
M0415B7 (See other available formats)
Regulatory Status
RUO
Other Names
Lipocalin 2, Oncogene 24p3, Neutrophil Gelatinase-Associated Lipocalin, Siderocalin LCN2, 25 kD Alpha-2-Microglobulin-Related Subunit Of MMP-9, p25, MSFI, Migration-Stimulating Factor Inhibitor, HNL
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
M0415B7_Purified_Antibody_WB_082714.jpg
Western blot analysis of HT-29 (lane 1), PBMC (lane 2), Raji (lane 3), Aspc-1 (lane 4), mouse bone marrow (lane 5), mouse spleen (lane 6) and human neutrophil (lane 7) using anti-NGAL antibody (clone M0415B7). Anti-GAPDH antibody (poly6314) was used as a loading control.
  • M0415B7_Purified_Antibody_WB_082714.jpg
    Western blot analysis of HT-29 (lane 1), PBMC (lane 2), Raji (lane 3), Aspc-1 (lane 4), mouse bone marrow (lane 5), mouse spleen (lane 6) and human neutrophil (lane 7) using anti-NGAL antibody (clone M0415B7). Anti-GAPDH antibody (poly6314) was used as a loading control.
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661902 100 µg £133
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Description

NGAL (lipocalin-2) is a lipocalin family protein that plays important roles in innate immunity, inflammation, and atherosclerosis. It was originally identified as a 25 kD protein covalently bound to gelatinase from human neutrophils. NGAL is a critical component of innate immunity because of its ability to bind iron-siderophore complexes and limit bacterial growth. It is also produced by injured epithelia and is one of the most promising markers for renal epithelial injury and cardiovascular disease (CVD).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human NGAL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone M0415B7 does not cross-react with mouse NGAL.

RRID
AB_2564180 (BioLegend Cat. No. 661902)

Antigen Details

Structure
193 amino acid monomer with a predicted molecular weight of approximately 22 kD.
Distribution
Secreted by epithelial cells, macrophages, neutrophils; increased levels have been observed in plasma, serum, and urine in various conditions, such as metastatic breast and colorectal cancer, acute kidney injury, pancreatitis and preeclampsia.
Function
Apoptosis, innate immunity, renal development.
Interaction
Disulfide-linked with MMP9.
Ligand/Receptor
SLC22A17 (24p3R)
Cell Type
Epithelial cells, Macrophages, Neutrophils
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Iqbal N, et al. 2013. Expert Opin. Med Diagn. 7:209.
2. Singer E, et al. 2013. Acta. Physiol. (Oxf) 207:663.
3. Palazzuoli A, et al. 2012. Clin. Chim. Acta. 413:1832.
4. Flo TH, et al. 2004. Nature 432:917.
5. Goetz DH, et al. 2002. Mol. Cell. 10:1033.
6. Leung L, et al. 2012. PLoS One 7:e46677.
7. Kjeldsen L, et al. 1993. J. Biol. Chem. 268:10425.

Gene ID
3934 View all products for this Gene ID
UniProt
View information about NGAL on UniProt.org
Go To Top Version: 0    Revision Date: 08/29/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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