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Purified anti-NMDAR1 Antibody (Previously Covance catalog# MMS-501P)

Pricing & Availability
Clone
R1JHL (See other available formats)
Regulatory Status
RUO
Other Names
GluN1, Glutamate [NMDA] receptor subunit zeta-1, NMD-R1, glutamate [NMDA] receptor subunit zeta 1, N-methyl-D-aspartate receptor subunit NR1, N-methyl-D-aspartate receptor channel, subunit zeta-1
Previously
Covance Catalog# MMS-501P
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
R1JHL_Purified_NMDAR1_Antibody_081519
Western blot of purified anti-NMDAR1 antibody (clone R1JHL). Lane 1: Molecular weight marker; Lane 2: 30 µg of rat brain lysate. The blot was incubated with 0.3 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • R1JHL_Purified_NMDAR1_Antibody_081519
    Western blot of purified anti-NMDAR1 antibody (clone R1JHL). Lane 1: Molecular weight marker; Lane 2: 30 µg of rat brain lysate. The blot was incubated with 0.3 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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828201 15 µg £254
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Description

Glutamate activated Ion channels that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDA receptor plays an essential role in the induction of LTP in the CA1 and dentate areas of the hippocampus as the specific NMDA antagonist, APV blocks LTP in these areas. This receptor has also been linked to neuronal development and it has been implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a fusion protein from aa1-564 of rat NMDA NR1. It is sold as lyophilized TCS.
Formulation
Lyophilized. Stored in DMEM + 10% FBS.
Preparation
Lyophilized from hybridoma tissue culture media.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.3 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Reconstitute in 50 ul of distilled water. For immunoblot dilute in TBST (20 mM Tris/HCl (7.6), 137 mM NaCl, 0.1% tween-20) containing 5% nonfat milk and 0.05% NaN3.

Product Citations
  1. Washburn HR, et al. 2020. Nat Commun. 11:570. PubMed
RRID
AB_2564918 (BioLegend Cat. No. 828201)

Antigen Details

Structure
Expected MW: 120 kD
Cell Type
Glutamatergic Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References
  1. Moriyoshi K, et al. 1991. Nature. 354:31.
  2. Monyer H, et al. 1992. Science. 256:1217.
  3. Ishii T, et al. 1993. J Biol Chem. 268:2836.
  4. Stephenson FA. 2001. Curr Drug Targets. 2:233. (IP)
  5. Louis JV, et al. 2011. Proc Natl Acad Sci U S A. 108:6957. (IHC)
Gene ID
2902 View all products for this Gene ID
UniProt
View information about NMDAR1 on UniProt.org
Go To Top Version: 4    Revision Date: 02/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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