Purified anti-NMDAR2B Antibody anti-NMDAR2B

Pricing & Availability

Clone: N59/20 (See other available formats)
Regulatory Status: RUO
Other Names: GluN2B/NR2B glutamate receptor, Glutamate receptor ionotropic, NMDA 2B, NR2B, NMDAR2B, glutamate receptor, ionotropic, NMDA2B, N-methyl D-aspartate receptor subtype 2B, glutamate [NMDA] receptor subunit epsilon-2
Previously: Covance Catalog# MMS-5149
Isotype: Mouse IgG2a, κ
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Product Citations: publications

N59-20_PURE_NMDAR2B_WB_113018 — Western blot of purified anti-NMDAR2B antibody (clone N59/20). Lane 1: Molecular weight marker; Lane 2: 5 µg of mouse brain membrane lysate; Lane 3: 10 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.

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832501

100 µL

£113

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Description

Glutamate activated Ion channels that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDA receptor plays an essential role in the induction of LTP in the CA1 and dentate areas of the hippocampus as the specific NMDA antagonist, APV blocks LTP in these areas. This receptor has also been linked to neuronal development and it has been implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against a fusion protein corresponding to amino acids 20-271 (extracellular, N-terminus) of rat GluN2B/NR2B glutamate receptor protein.
Formulation: Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2564957 (BioLegend Cat. No. 832501)

Antigen Details

Structure: Expected MW: 170 kD
Cell Type: Glutamatergic Neurons
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family: Postsynaptic proteins
Gene ID: 24410 View all products for this Gene ID
UniProt: View information about NMDAR2B on UniProt.org

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Western Blotting Protocol

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Other Formats

View All NMDAR2B Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-NMDAR2B	N59/20	WB
HRP anti-NMDAR2B	N59/20	WB,IHC-P

Certificate of Analysis

Go To Top Version: 5 Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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