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Purified anti-Notch 1 Antibody (Previously Covance catalog# MMS-5169)

Pricing & Availability
Clone
N253/32 (See other available formats)
Regulatory Status
RUO
Other Names
Neurogenic locus notch homolog protein 1, mT14, p300, Motch A, Notch gene homolog 1, major type A protein, transmembrane receptor Notch1, N1, Mis6, Tan1, lin-12, 9930111A19Rik, MMS-5169-100
Previously
Covance Catalog# MMS-5169
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
1-N253slash32_Purified_Notch_1_Antibody_NPRF_022318
Western blot of purified anti-NOTCH 1 antibody (clone N253/32). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain membrane; Lane 3: 30 µg of mouse brain membrane lysate; Lane 4: 30 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1-N253slash32_Purified_Notch_1_Antibody_NPRF_022318
    Western blot of purified anti-NOTCH 1 antibody (clone N253/32). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain membrane; Lane 3: 30 µg of mouse brain membrane lysate; Lane 4: 30 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 2-N253slash32_Purified_Notch_1_Antibody_IHC_030415
    IHC staining of purified anti-NOTCH 1 antibody (clone N253/32) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
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819101 100 µL £113
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Description

Notch 1, also known as TAN-1, is a transmembrane protein. Its extracellular domain contains 29 epidermal growth factor-like (EGF) repeats and 3 Lin/Notch Glp (LNR) repeats, the intracellular domain contains 5 CDC10/Ankryn repeats (ANK), 1 proline, glutamate, serine, threonine-rich (PEST) motif, and 1 regulation of amino acid metabolism 23 (RAM23) domain. Notch 1 regulates the development, differentiation, and survival of a broad spectrum of cell lineages. It is involved in myogenesis, neurogenesis, gliogenesis, and lymphocyte development, resulting in Notch 1 expression in many organs such as brain, lung, thymus, spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney, and heart. Notch 1 ligands are Jagged 1, Jagged 2, Delta 1, and Delta 4. Upon ligand binding, the intracellular domain of Notch 1 is cleaved and translocates to the cell nucleus where it forms a transcriptional activator complex with RBP-J κ.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration of 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is less reactive in human tissue in IHC-P.

Product Citations
  1. Bohl B, et al. 2022. Sci Adv. 8:eabl5792. PubMed
RRID
AB_2564827 (BioLegend Cat. No. 819101)

Antigen Details

Structure
Expected molecular weight: >270 kD, 120 kD
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
18128 View all products for this Gene ID
UniProt
View information about Notch 1 on UniProt.org
Go To Top Version: 4    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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