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Purified anti-NR5A2 Antibody

Pricing & Availability
Clone
1D12B67 (See other available formats)
Regulatory Status
RUO
Other Names
Nuclear receptor subfamily 5 group A member 2, orphan nuclear receptor NR5A2, HB1F, fetoprotein transcription factor, FTF, CPF, CYP7A promoter binding factor, LRH1,B1F2, FTZ-F1, hB1F-2, FTZ-F1beta
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1D12B67_PURE_NR5A2_Antibody_WB_100115
Total cell lysate from human HepG2 cells (lane 1) and mouse liver cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-NR5A2 (clone 1D12B67) antibody. Proteins were visualized using an HRP-conjugated anti-mouse-IgG secondary and chemiluminescence detection.
  • 1D12B67_PURE_NR5A2_Antibody_WB_100115
    Total cell lysate from human HepG2 cells (lane 1) and mouse liver cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-NR5A2 (clone 1D12B67) antibody. Proteins were visualized using an HRP-conjugated anti-mouse-IgG secondary and chemiluminescence detection.
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681202 100 µg £230
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Description

The nuclear receptor subfamily 5 group A member 2, also known as NR5A2, is a 54 kD nuclear receptor protein. NR5A2 is a member of the FTZ-F1 sub-family and is highly expressed in the pancreas and to a lesser extent in the liver. NR5A2 contains a DNA binding domain, a hormone ligand-binding domain, and two zinc finger domains. It also binds to DNA as a monomer. NR5A2 is known to regulate the transcription of the liver enzyme cholesterol 7-alpha-hydrolase, which converts cholesterol to bile acids and also activates the hepatitis B virus enhancer II to contribute to the liver-specific expression of this virus. NR5A2 has been suggested to regulate pancreas-specific gene expression and to play a role in embryonic development. There are at least 3 alternatively spliced isoforms of NR5A2: isoforms 1 and 2 are present in fetal and adult liver and in liver cancer cells HepG2 as well.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human NR5A2 recombinant protein (1-200 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2566455 (BioLegend Cat. No. 681202)

Antigen Details

Structure
Orphan nuclear receptor, member of the FTZ-F1 sub-family, contains a DNA binding domain, 2 zinc finger motifs, a FTZ-F1 box, and a ligand-binding domain. Approximately 54 kD, three alternatively spliced variants exist.
Distribution
Nuclear, highly expressed in the pancreas, lower expression in the liver, and very low expression in the heart and lungs.
Function
Binds DNA as a monomer to regulate the transcription of the liver enzyme cholesterol 7-alpha-hydroxylase, which converts cholesterol to bile acids. Activates viral hepatitis B enhancer II to regulate liver-specific expression of this virus in combination with other transcription factors.
Interaction
Interacts wtih GRIP1, NCOA2, and NR0B2.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Li M, et al. 1998. J. Biol. Chem. 273:29022.
2. Nitta M, et al. 1999. Proc. Natl. Acad. Sci. USA 96:6660.
3. Lee YK, et al. 2002. J. Biol. Chem. 277:2463.
4. Zhang CK, et al. 2001. Gene 273:239.

Gene ID
2494 View all products for this Gene ID
UniProt
View information about NR5A2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10/01/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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