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Purified anti-NRAS Antibody

Pricing & Availability
Clone
A18072A (See other available formats)
Regulatory Status
RUO
Other Names
NRAS Proto-Oncogene, GTPase NRas, Neuroblastoma RAS Viral Oncogene Homolog
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A18072A_PURE_NRAS_Antibody_1_061819
Western blot of purified anti-NRAS antibody (clone A18072A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18072A_PURE_NRAS_Antibody_1_061819
    Western blot of purified anti-NRAS antibody (clone A18072A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18072A_PURE_NRAS_Antibody_2_061819
    IHC staining of purified anti-NRAS antibody (clone A18072A) on formalin-fixed paraffin-embedded mouse colon tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • A18072A_PURE_NRAS_Antibody_3_061819
    IHC staining of purified anti-NRAS antibody (clone A18072) on formalin-fixed paraffin-embedded rat colon tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
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866101 25 µg £73
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866102 100 µg £189
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Description

NRAS is a Ras gene family member originally identified in human neuroblastoma cells. Like other members of the Ras family, NRAS has GTP/GDP binding and GTPase activity, and functions as a G-like regulatory protein that plays a role in regulating cell growth. Mutations in NRAS have been shown to transform cultured cells to tumor cells, implicating its role in tumorigeneses, as potential therapeutic target for cancers such as melanoma.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human NRAS a.a. 1-186
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunohistochemistry, a concentration range of 2.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Lucas CD, et al. 2022. Sci Immunol. 7:eabm4032. PubMed
RRID
AB_2810783 (BioLegend Cat. No. 866101)
AB_2810783 (BioLegend Cat. No. 866102)

Antigen Details

Structure
NRAS is a 189 amino acid protein with predicted and observed molecular mass of ~ 21 kD.
Distribution

Tissue Distribution: Ubiquitously expressed. Highly expressed in appendix, gallbladder, and gastrointestinal tract.
Cellular Distribution: Plasma membrane, Golgi apparatus, cytosol, and nucleus.

Function
G-Like regulatory protein that plays a role in regulating cell growth.
Interaction
RGS14, RASSF7
Ligand/Receptor
GTP, GDP
Cell Type
Astrocytes, Endothelial cells, GABAergic Neurons, Glutamatergic Neurons, Mature Neurons, Microglia, Neural Stem Cells, Oligodendrocytes
Biology Area
Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Neuroscience, Stem Cells
Molecular Family
GTPases
Antigen References
  1. Rocks O, et al. 2005. Science. 307:1746-52.
  2. Byrne M, Saif MW. 2019. Onco. Targets Ther. S194605
  3. Sexton RE, et al. 2019. Semin. Cancer Biol. 54:131-7.
Gene ID
4893 View all products for this Gene ID 24605 View all products for this Gene ID
UniProt
View information about NRAS on UniProt.org
Go To Top Version: 1    Revision Date: 06/18/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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