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Purified anti-NSE Antibody (Previously Covance catalog# MMS-519P)

Pricing & Availability
Clone
NSE-P2 (See other available formats)
Regulatory Status
RUO
Other Names
Gamma-enolase, neural enolase, neuron-specific enolase, 2-phospho-D-glycerate hydrolyase, ENO2, NSE
Previously
Covance Catalog# MMS-519P
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
NSE-P2_Purified_NSE_Antibody_1_061218
IHC staining of purified anti-NSE antibody (clone NSE-P2) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • NSE-P2_Purified_NSE_Antibody_1_061218
    IHC staining of purified anti-NSE antibody (clone NSE-P2) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • NSE-P2_Purified_NSE_Antibody_2_061218
    IHC staining of purified anti-NSE antibody (clone NSE-P2) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • NSE-P2_Purified_NSE_Antibody_3_061218
    IHC staining of purified anti-NSE antibody (clone NSE-P2) on formalin-fixed paraffin-embedded human pancreas tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C . BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 50 µm
  • NSE-P2_Purified_NSE_Antibody_4_061218
    Western blot of purified anti-NSE antibody (clone NSE-P2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • NSE-P2_Purified_NSE_Antibody_5_022020
    ICC staining of purified anti-NSE antibody (clone NSE-P2) on SH-SY5Y Cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The cells were co-stained with Flash Phalloidin™ Green 488 (Cat. No. 424201). The slide was mounted with fluoromount G with DAPI. The image was captured with a 60X objective. Scale bar: 20 µm
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804501 200 µL £244
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Description

Neuron specific enolase is found in elevated concentrations in plasma and certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer.

Neuron specific enolase (NSE) converts 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers. NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. Neurons require a great deal of energy and have high levels of glycolytic enzymes such as NSE. Antibodies to this NSE are useful for identifications of neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a synthetic peptide corresponding to amino acids 271-285. It recognizes the sequence TGDQLGALYQDFVRD.
Formulation
Phosphate-buffered solution + 0.03% thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per mL. For immunocytochemistry, a concentration range of 0.5 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2564666 (BioLegend Cat. No. 804501)

Antigen Details

Structure
Expected MW: 47 kD
Cell Type
Mature Neurons, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Phospho-Proteins
Antigen References
  1. Murray GI, et al. 1993. J Clin Pathol. 46:993.
  2. Duncan ME, et al. 1992. J immunol Methods. 151:227
Gene ID
2026 View all products for this Gene ID
UniProt
View information about NSE on UniProt.org
Go To Top Version: 5    Revision Date: 02/20/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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