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Purified anti-Nucleophosmin Antibody

Pricing & Availability
Clone
P87H10B9 (See other available formats)
Regulatory Status
RUO
Other Names
NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
2_P87H10B9_PURE_Nucleophosmin_Antibody_2_WB_031519.png
Whole cell extracts (15 µg total protein) from Daudi, Molt-4, and HeLa were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.25 µg/mL (1:2000 dilution) of Purified anti-Nucleophosmin Antibody, clone P87H10B9 overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. Cell lysates were loaded in ascending order of predicted NPM1 expression; Predicted expression data was obtained from Human Protein Atlas.
  • 2_P87H10B9_PURE_Nucleophosmin_Antibody_2_WB_031519.png
    Whole cell extracts (15 µg total protein) from Daudi, Molt-4, and HeLa were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.25 µg/mL (1:2000 dilution) of Purified anti-Nucleophosmin Antibody, clone P87H10B9 overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. Cell lysates were loaded in ascending order of predicted NPM1 expression; Predicted expression data was obtained from Human Protein Atlas.
  • A_P87H10B9_PURE_Nucleophosmin_Antibody_1_WB_072116
    Total cell lysate from NIH3T3 cells (lane 1, 15 µg) and HeLa cells (lane 2, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-Nucleophosmin antibody (clone P87H10B9) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Purified anti-β-actin antibody (poly6221) was used as a loading control.
  • B_P87H10B9_PURE_Nucleophosmin_2_IF_032318
    HeLa cells were fixed with 2% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then, the cells were intracellularly stained with 1 μg/ml Purified anti-Nucleophosmin antibody (clone P87H10B9) overnight at 4°C followed by Alexa Fluor® 594 (red) conjugated goat anti-mouse IgG for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 40x objective.
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686802 100 µg £238
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Description

Nucleophosmin is a member of the nucleophosmin/nucleoplasmin family that shuttles between the nucleus and cytoplasm. This protein regulates the stability and transcriptional activity of p53 and acts as a molecular chaperone. It preferentially binds to denatured proteins and has been shown to stimulate DNA polymerase activity and control the duplication of centrosomes. The chaperone activity of nucleophosmin is regulated by protein kinase CK2 and promotes the release of denatured substrates from nucleophosmin. It is modified by phosphorylation on Thr199. This protein has been shown to bind to RNA and DNA and interact with nucleolar proteins including nucleolin, protein P120, HIV-1 Rev protein, and hepatitis delta antigens.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant human nucleophosmin (2-265 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2616887 (BioLegend Cat. No. 686802)

Antigen Details

Structure
294 amino acids with a predicted molecular weight of 33 kD.
Distribution

Nucleolus.

Function
Involved in ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF.
Interaction
NSUN2, SENP3, hepatitis delta virus S-HDAg, HTLV1 Rex protein, methylated form of RPS10, APEX1, NEK2, ROCK2 and BRCA2, RPGR, CENPW, EIF2AK2/PKR, CEBPA (isoform 4), and DDX31.
Biology Area
Cell Biology
Molecular Family
Phospho-Proteins
Antigen References

1. Szebeni A, et al. 2003. J. Biol. Chem. 278:9107.
2. Colombo E, et al. 2002. Nat. Cell Biol. 4:529.
3. Chan PK, et al. 1986. J. Biol. Chem. 261:14335.

Gene ID
4869 View all products for this Gene ID
UniProt
View information about Nucleophosmin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 03/15/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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