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Purified anti-O-GlcNAc Antibody

Pricing & Availability
Clone
RL2 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
RL2_PURE_O-GlcNAc_Antibody_WB_062215
Same amount (15 µg) of total protein harvested from HeLa, NIH3T3, rat E18 brain, and mouse brain were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal O-GlcNAc (clone RL2) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • RL2_PURE_O-GlcNAc_Antibody_WB_062215
    Same amount (15 µg) of total protein harvested from HeLa, NIH3T3, rat E18 brain, and mouse brain were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal O-GlcNAc (clone RL2) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
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677902 100 µg £238
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Description

The monoclonal antibody RL2 reacts specifically with beta-O-linked N-acetylglucosamine (O-GlcNAc). It recognizes o-GlcNAc in beta O-glycosidic linkage to both serine and threonine. However, it exhibits no cross reactivity with alpha-linked Ser-O-linked N-acetylgalactosamine or N-linked oligosaccharides on ovalbumin and immunoglobulin. This antibody readily detects in vivo changes in O-GlcNAc modification1.

O-GlcNAc is one of the most abundant posttranslational modifications within the nucleocytoplasmic compartments of all metazoans2. O-GlcNAcylation is similar to protein phosphorylation. Research on O-GlcNAc is particularly relevant to chronic human diseases such as diabetes, cardiovascualr disease, neurodegenerative disorders, and cancer3.

Like phosphorylation, the specific functions of O-GlcNAc depend on the protein and sites to which the glycan is attached2. One of the major functions of O-GlcNAc is to prevent O-phosphorylation and to modulate signaling and transcription in response to cellular nutrients or stress4.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Reported Reactivity
Other species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
O-GlcNAc in beta-O-glycosidic linkage to both serine and threonine.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Snow CM, et al. 1987. J. Cell Biol. 104:1143.
  2. Shin SH, et al. 2011. Amino Acids 40:885-93.
  3. Mi W, et al. 2011. Biochim. Biophys. Acta. 4:514.
  4. Hoedt E, et al. 2010. J. Biol. Chem. 285:19205.
Product Citations
  1. Upadhyay D, et al. 2024. STAR Protoc. 102817:5. PubMed
  2. Wang Z, et al. 2020. Theranostics. 5.401388889. PubMed
  3. Li R, et al. 2021. Shock. 56:755. PubMed
RRID
AB_2565912 (BioLegend Cat. No. 677902)

Antigen Details

Structure
O-GlcNAc modified protein.
Distribution

Nucleus and cytoplasm.

Function
To prevent O-phosphorylation and to modulate signaling and transcription in response to cellular nutrients or stress.
Biology Area
Cell Biology, Signal Transduction, Transcription Factors
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Comer FI, et al. 2001. Anal Biochem. 293:169.
2. Hart, GW and Akimoto, Y. (2009) Essentials of Glycobiology, 2nd edition. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
3. de Queiroz RM, et al. 2014. Front Oncol. 4:132.
4. Hart GW, et al. 2014. Cell Metab. 20:208.

Gene ID
8473 View all products for this Gene ID
UniProt
View information about O-GlcNAc on UniProt.org
Go To Top Version: 4    Revision Date: 09/22/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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