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Purified anti-Oct-1 (POU2F1) Antibody

Pricing & Availability
Clone
W18054D (See other available formats)
Regulatory Status
RUO
Other Names
POU domain, class 2, transcription factor 1, Octamer-binding transcription factor 1, NF-A1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18054D_PURE_Oct-1_Antibody_1_071519
Total cell lysates (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:5000 dilution) of Purified anti-Oct-1 (POU2F1) Antibody, clone W18054D, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker
  • W18054D_PURE_Oct-1_Antibody_1_071519
    Total cell lysates (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:5000 dilution) of Purified anti-Oct-1 (POU2F1) Antibody, clone W18054D, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker
  • W18054D_PURE_Oct-1_Antibody_2_071519
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.0 µg of Purified Rat IgG2a, κ Isotype control antibody (Cat. No. 400502) or Purified anti-Oct-1 (POU2F1) Antibody, clone W18054D. The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a rabbit control antibody against a separate epitope of Oct-1. Lane M: Molecular weight marker
Cat # Size Price Quantity Check Availability Save
625701 25 µg £70
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625702 100 µg £174
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Description

Oct-1 is a member of the Pit-Oct-Unc (POU) domain family of transcription factors, which are characterized by a bipartite POU domain. The POU domain that contains two DNA-binding subdomains; an N-terminal POU-specific subdomain and a C-terminal homeobox subdomain, each of which are tethered by a variable linker sequence. Oct-1 can function as both a transcriptional activator and repressor, and controls the expression of housekeeping genes such as those encoding Histone H2B and snRNPs, as well as genes involved in development and differentiation. The highly-modular POU domain enables Oct-1 to drive diverse transcriptional programming that occurs in a tissue-specific manner. The POU domains confers the flexibility for Oct-1 to function as both a monomer, dimer, and heterodimer, and also facilitates the interaction between Oct-1 with a broad set of transcriptional cofactor proteins. Moreover, Oct-1 undergoes multiple post-translational modifications that confers DNA-binding specificity to certain promoters, including phosphorylation, ubiquitination, and sumoylation. Oct-1 is highly-expressed in many types of cancers, and functionally different isoforms produced from alternative promoter usage have been reported.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant protein corresponding to human OCT-1 amino acid residues 251-500
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was not tested for ICC

Non-specific band present in Ramos at 60 kD

RRID
AB_2814492 (BioLegend Cat. No. 625701)
AB_2814492 (BioLegend Cat. No. 625702)

Antigen Details

Structure
Oct-1 is a 743 amino acid protein with a predicted molecular weight of 76kD
Distribution

Nucleoplasm/Ubiquitously expressed

Function
Transcription factor
Biology Area
Cell Biology, Transcription Factors
Antigen References
  1. Pankratova EV, et al. 2018. Oncotarget. 9:29892
  2. Strum RA and Herr W. et al. 1988. Nature. 336:601.
  3. Strum RA, et al. 1988. Genes Dev. 2:1582.
Gene ID
5451 View all products for this Gene ID
UniProt
View information about Oct-1 on UniProt.org
Go To Top Version: 1    Revision Date: 07/15/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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