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Purified anti-Olig2 Antibody

Pricing & Availability
Clone
W21023A (See other available formats)
Regulatory Status
RUO
Other Names
Oligodendrocyte transcription factor 2 (Olig2), Class B basic helix-loop-helix protein 1 (bHLHb1), Class E basic helix-loop-helix protein 19 (bHLHe19), Protein kinase C-binding protein 2 (PRKCBP2), RACK17, OLIGO2
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A_W21023A_PURE_Olig2_IHC_Antibody_060123
IHC staining of purified anti-Olig2 (clone W21023A) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated without (panel A) and with (panel B) 5 μg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 μm
  • A_W21023A_PURE_Olig2_IHC_Antibody_060123
    IHC staining of purified anti-Olig2 (clone W21023A) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated without (panel A) and with (panel B) 5 μg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 μm
  • B_W21023A_PURE_Olig2_ICC_Antibody_060123
    Rat cortical stem cells induced to differentiate via FGF withdrawal were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 5 μg/mL of purified anti-Olig2 (clone W21023A), followed by incubation with Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 60X objective. Scale bar: 50 μm
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615951 25 µg £110
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615952 100 µg £274
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Description

Olig2 is a transcription factor that plays a crucial role in the development and maintenance of the central nervous system. This transcription factor is a master regulator for oligodendrocyte cell specification. Olig2 activates the expression of myelin-associated genes in oligodendrocyte lineage cells, which are essential for proper neuronal function and transmission of nerve impulses. In addition to its role in oligodendrocyte differentiation and maturation, Olig2 also regulates the development and maintenance of motor neurons in the spinal cord and contributes to the proliferation and differentiation of neural stem cells. Dysregulation of Olig2 expression has been associated with several neurological disorders, including multiple sclerosis and gliomas, highlighting its importance in maintaining normal brain function. Being located on chromosome 21, dysregulation of Olig2 is also associated with Down Syndrome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of the N-terminus of mouse Olig2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product is not recommended for use in Western Blot. Although a band of the correct size was observed when testing in human, mouse and rat brain, there are multiple non-specific bands.

This product does not recognize mouse Olig2 by IHC-F.

For IHC-P, we recommend antigen retrieval using Tris-EDTA (10mM Tris, 1mM EDTA, 0.05% Tween-20, pH 9.0).

For ICC, we recommend either 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilization with 0.5% Triton X-100 for 10 minutes, or 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilization with 100% ice-cold methanol for 10 minutes.

RRID
AB_2941623 (BioLegend Cat. No. 615951)
AB_2941623 (BioLegend Cat. No. 615952)

Antigen Details

Structure
Olig2 is a 323 amino acid protein with a predicted molecular weight of 32.38 kD.
Distribution

Nucleus

Function
Transcription factor
Cell Type
Neural Stem Cells, Oligodendrocytes
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Antigen References
  1. Zhang K, et al. 2022. Nat Commun. 13:1423.
  2. Xu R, et al. 2019. Cell Stem Cell. 24:908-926.
  3. Sock E, Wegner M. 2021. Dev Neurobiol. 81:892-901. 
  4. Szu J, et al. 2021. Front Neurosci. 15:659601.
Gene ID
10215 View all products for this Gene ID
UniProt
View information about Olig2 on UniProt.org
Go To Top Version: 1    Revision Date: 06/01/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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