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Purified anti-Oligodendrocyte Marker O4 Antibody

Pricing & Availability
Clone
O4 (See other available formats)
Regulatory Status
RUO
Other Names
Galactocerebroside O4, Oligodendrocyte marker O4, Oligodendrocyte O4 antigen, Sulfated Galactocerebroside O4
Isotype
Mouse IgM, κ
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Product Citations
publications
O4_PURE_Oligodendrocyte-Marker-O4_Antibody_112322
IHC staining using purified anti-Oligodendrocyte marker O4 (clone O4) on formalin-fixed paraffin-embedded human cerebellum tissue. The tissue was incubated overnight at 4°C with 0.5 μg/mL of anti-Oligodendrocyte marker O4 (panels A and B) or without primary antibody (panels C and D), followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgM (red) (Cat. No. 406526) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade mountant reagent. The images were captured with a 40X objective. Panels A and C are merged images of DAPI and Alexa Fluor® 647 channels, and panels B and D are images showing only Alexa Fluor® 647 channel. Scale bar = 50 μm
  • O4_PURE_Oligodendrocyte-Marker-O4_Antibody_112322
    IHC staining using purified anti-Oligodendrocyte marker O4 (clone O4) on formalin-fixed paraffin-embedded human cerebellum tissue. The tissue was incubated overnight at 4°C with 0.5 μg/mL of anti-Oligodendrocyte marker O4 (panels A and B) or without primary antibody (panels C and D), followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgM (red) (Cat. No. 406526) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade mountant reagent. The images were captured with a 40X objective. Panels A and C are merged images of DAPI and Alexa Fluor® 647 channels, and panels B and D are images showing only Alexa Fluor® 647 channel. Scale bar = 50 μm
  • O4_PURE_Oligodendrocyte-Marker-O4_Antibody_2_112122
    Differentiated rat cortical stem cells were fixed with 4% paraformaldehyde and permeabilized with methanol. Cells were intracellularly stained with 5.0 µg/mL of purified anti-Oligodendrocyte marker O4 (clone O4) overnight at 4°C, followed by incubation with Alexa Fluor® 647 anti-mouse IgM (Cat. No. 406526) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective. Scale bar: 50 μm
  • O4_PURE_Oligodendrocyte-Marker-O4_Antibody_3_112322
    Differentiated rat cortical stem cells were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and stained with purified anti-Oligodendrocyte marker O4 antigen (clone O4) (blue dot plot), or mouse IgM, κ isotype control (Cat. No. 401602) (red dot plot), followed by Alexa Fluor® 647 anti-mouse IgM (Cat. No. 406526). Left density plot (FSC-A vs. SSC-A) shows the gated population (stem cells and complex cells, and right dot plots (Alexa Fluor® 647 vs. FSC-A) shows stain intensity for each specific gated population.
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609551 25 µg £107
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609552 100 µg £310
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Description

Oligodendrocytes are the myelinating cells of the central nervous system (CNS). Similar to the Schwann cells in the peripheral nervous system (PNS), their function is to provide support and isolation to neuronal axons. Their importance has been denoted on de-myelinating diseases such as multiple sclerosis, amyotrophic lateral sclerosis, periventricular leukomalacia, and also, they have been associated with dementia, schizophrenia, cognitive impairment, and bipolar disorder. Oligodendrocytes develop during embryogenesis; however, a small proportion of progenitor stem cells are present in the adult brain. Thus, three stages of differentiation can be identified in the cerebral white matter, and specific markers have been associated which each particular stage. Sulfated Galactocerebroside O4 (marker O4) is a major galactosphingolipid component of oligodendrocyte plasma membrane. It is produced from early immature non-myelinating oligodendrocytes to mature myelinating oligodendrocytes, and together with expression of other markers, it has been widely used as characteristic marker for these specific differentiation stages.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
White matter from bovine corpus callosum (from developer)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on testis samples, a concentration range of 1.25 - 5.0 µg/mL is suggested. For immunohistochemistry on cerebellum samples, a concentration range of 0.5 - 1.25 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. 

Application Notes

Clone O4 recognizes specifically O4 antigen by immunocytochemistry in oligodendrocytes derived from cortical stem cells using different fixation/permeabilization methods such as methanol, PFA/Triton X-100, and PFA/methanol, where the last method shows the highest signal intensity.

Following immunohistochemistry in paraffin sections, clone O4 showed specific high staining on cerebellum white matter, and very low to none staining in the molecular layer at the recommended concentration.

By flow cytometry staining on differentiated cortical stem cells, clone O4 stained at high intensity complex cells (oligodendrocytes) compared with low complexity cells (undifferentiated progenitor stem cells).

RRID
AB_2927990 (BioLegend Cat. No. 609551)
AB_2927990 (BioLegend Cat. No. 609552)

Antigen Details

Structure
It is a sulfated galactocerebroside (glycosphingolipid), composed of a single galactose linked to a ceramide
Distribution

Present in the plasma membrane of early immature to mature oligodendrocytes

Cell Type
Neural Stem Cells, Oligodendrocytes
Biology Area
Neurodegeneration, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Bansal R, et al. 1989. J Neurosci Res. 24:548-57.
  2. Bansal R, et al. 1999. J Neurosci. 19:7913-24.
  3. Ahmad I, et al. 2021. PloS One. 16:e0256155.
  4. Rasti BF, et al. 2020. Int J Nanomedicine. 15:3903-3920.
Gene ID
NA
UniProt
View information about Oligodendrocyte Marker O4 on UniProt.org
Go To Top Version: 1    Revision Date: 11/21/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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