- Clone
- W22006E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Cyclin Dependent Kinase Inhibitor 1A, CAP20, CIP1, WAF1, SDI1, p21, p21 CIP1, CDKN1, p21 Cip1/Waf1
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
645951 | 25 µg | £113 | ||||
645952 | 100 µg | £336 |
p21 (also known as CIP1 or CDKN1A) is a G1 cell cycle regulator that inhibits cyclin-cyclin dependent kinase 2 and/or cyclin-cyclin dependent kinase 4 complexes. Its expression is highly controlled by the tumor suppressor protein p53, thus, p21 is involved in controlling the G1 cell cycle arrest in response to different stress stimuli. p21 plays a regulatory role in S phase DNA replication by interacting with PCNA (proliferating cell nuclear antigen). Activation of cyclin-dependent kinase 2 has been observed after p21 is cleaved by CASP3-like caspases. Loss of function of p21 has been associated with bladder cancer, Multiple Endocrine Neoplasia Type I and lymph node metastasis. In addition, p21 can bind the HIV integrase mediating resistance to infection in hematopoietic cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant protein fragment of p21
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P, ICC, ICFC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For use of this antibody (clone W22006E) in immunocytochemistry (ICC), we recommend fixation/permeabilization with either PFA/Triton X-100 or PFA/Methanol. This antibody is not suitable for use with methanol fixation/permeabilization.
- RRID
-
AB_3662327 (BioLegend Cat. No. 645951)
AB_3662327 (BioLegend Cat. No. 645952)
Antigen Details
- Structure
- p21 is a 164 amino acid length protein with an approximate molecular weight of 18 kD.
- Distribution
-
Nuclear localization; in some diseases, it is localized in the cytoplasm.
- Function
-
Control of cell cycle progression
Inhibition of cyclin-dependent kinase activity
Inhibits DNA synthesis by competing with POLD3
Play a role in p53 inhibition of cell proliferation in response to DNA damage - Interaction
- Nrf2, BCCIP, CIZ1, CUL4A, CCNE1, CDK, DDB1, DTL, GADD45A, GADD45G, HDAC, PCNA, PIM1, TK1, TSG101
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability
- Molecular Family
- Tumor Suppressors
- Antigen References
-
- Sorteberg AL, et al. 2022. Front Oncol. 12:906194.
- Galanos P, et al. 2016. Cell Cycle. 23:3153-3154.
- Lai L, et al. 2020. Int J Mol Sci. 21:8504.
- Xiao BD, et al. 2020. World Stem Cells. 12:481.
- Gene ID
- 1026 View all products for this Gene ID
- UniProt
- View information about p21 on UniProt.org
Other Formats
View All p21 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-p21 | W22006E | WB,IHC-P,ICC,ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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