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Purified anti-p53 Antibody

Pricing & Availability
Clone
DO-1 (See other available formats)
Regulatory Status
RUO
Other Names
Tumor protein p53 (TP53)
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_DO-1_Purified_p53_Antibody_031519_updated.png
Whole cell extracts (15 µg total protein) from HEK293, A431, and THP-1 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to PVDF, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-p53 Antibody, clone DO-1, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Cell lysates were loaded in descending of predicted p53 expression; Predicted expression data was obtained from Human Protein Atlas.
  • 1_DO-1_Purified_p53_Antibody_031519_updated.png
    Whole cell extracts (15 µg total protein) from HEK293, A431, and THP-1 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to PVDF, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-p53 Antibody, clone DO-1, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Cell lysates were loaded in descending of predicted p53 expression; Predicted expression data was obtained from Human Protein Atlas.
  • 2_DO-1_WB_05/11/09_2
    Immunofluorescence of Daudi cells with (A) Mouse IgG2b, κ Isotype control (Cat. No. 400302) or (B-D) TP-53 primary antibody. Alexa Fluor® 594 (Red) Goat anti-Mouse IgG (Cat. No. 405326) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective. Exposure time (Seconds) is 1/12. Concentrations for (A, B) is 5 µg/ml, (C) is 2 µg/ml, (D) is 1 µg/ml.
  • 3_DO-1_ChIP_p53_Antibody_030320_updated.png
    Chromatin Immunoprecipitations (ChIP) were done with fixed and sonicated chromatin samples from 293T cells treated with tunicamycin (2 µg/mL, 8 hr). ChIP was performed with 5 µg of chromatin and either 1 µg of Go-ChIP-Grade™ purified anti-p53 antibody (Clone DO-1), or equal amount of Go-ChIP-Grade™ purified mouse IgG2a, κ isotype ctrl antibody (Clone MG2a-53, Cat. No. 401506). The enriched DNA was purified and quantified by real-time qPCR using SYBR Green and primers for the human CDKN1A promoter, and for a gene desert region on human chromosome 12. The amount of immunoprecipitated DNA in each sample is represented as a percentage of the total amount of input chromatin, which is equivalent to 100%.
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645701 25 µg £73
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645702 100 µg £155
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Description

p53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
P53 N-terminal linear epitope aa 20-25
Formulation
This antibody is provided in phosphate-buffered solution containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ChIP - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5 - 1 µg per ml antibody for each mini-gel. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/ml (Dilution 1:100 - 1:250) is recommended. For ChIP applications, the recommended starting antibody to chromatin ratio is 1 µg of antibody and 5 µg of chromatin per IP. Optimal antibody to chromatin ratio should be determined by the end user. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Vojtesek B, et al. 1992. J Immuno Methods 151:237
  2. Stephen CW, et al. 1995. J Mol Biol 248:58
Product Citations
  1. Piccini I, et al. 2022. Br J Dermatol. 186:129. PubMed
  2. Geisinger JM, et al. 2020. Nucleic Acids Res. 48:9067. PubMed
  3. Linetsky M, et al. 2020. Free Radic Biol Med. 152:280. PubMed
  4. Lim J, et al. 2021. Curr Protoc. 1:e29. PubMed
RRID
AB_1595606 (BioLegend Cat. No. 645701)
AB_1595606 (BioLegend Cat. No. 645702)

Antigen Details

Structure
Tetramer; 53 kD
Distribution

Nuclear when activated

Function
Tumor suppressor, transcription factor regulates cell cycle checkpoint, apoptosis, DNA integrity
Interaction
mdm2, mdmx, TopoI, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, HDAC1-3; mutant p53 associates with p63, p73
Cell Type
Mesenchymal Stem Cells
Biology Area
Cell Biology, DNA Repair/Replication, Stem Cells, Transcription Factors
Antigen References

1. Vogelstein B, et al. 1992. Cell. 70:523.
2. Shieh S, et al. 1997. Cell. 91:325.
3. Mihara M, et al. 2003. Mol. Cell 11:577.
4. Saito S, et al. 2003. J. Biol. Chem. 278:37536.

Gene ID
7157 View all products for this Gene ID
UniProt
View information about p53 on UniProt.org
Go To Top Version: 5    Revision Date: 08/24/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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