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Purified anti-p63 (ΔN) Antibody

Pricing & Availability
Clone
W17048F (See other available formats)
Regulatory Status
RUO
Other Names
p63, δN p63-α, p63-β, p63-γ, p63-δ, TP63, tumor protein p63
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17048F_Pure_p63_Antibody_1_121319_updated.png
Whole cell extracts (15 µg protein) from HeLa (negative control), ME-180 and HaCaT (positive controls) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-p63 (ΔN) antibody (clone W17048F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. Cell lysates were loaded in order of ascending TP63 expression; predicted expression data was obtained from Human Protein Atlas.
  • W17048F_Pure_p63_Antibody_1_121319_updated.png
    Whole cell extracts (15 µg protein) from HeLa (negative control), ME-180 and HaCaT (positive controls) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-p63 (ΔN) antibody (clone W17048F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. Cell lysates were loaded in order of ascending TP63 expression; predicted expression data was obtained from Human Protein Atlas.
  • W17048F_Pure_p63_Antibody_2_121319_updated.png
    HaCaT cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 5.0 µg/mL (1:500 dilution) of either purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400502, panel A) or purified anti-p63 (ΔN) antibody (panel B) overnight at 4°C, followed by incubation with Alexa Fluor™ 488 goat anti-rat IgG (Cat. No. 405418) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
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699501 25 µg £70
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699502 100 µg £174
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Description

p63 (ΔN) is a member of the p53 family. This protein has multiple isoforms ΔN p63-α, -β, and -γ. The molecular weights of the p63 isoforms are: α=66 kD, β=52 kD, and γ=45 kD. This nuclear protein is essential for limb formation, carcinogenesis, epidermal morphogenesis, and epithelial stem cell regeneration. ΔN isoforms are antagonistic to p53; TA isoforms can transactivate p53 targets. The p63 ΔN isoforms repress TA isoforms; mutant p53 abrogates p63 transactivation. This protein interacts with p53, p73, and the various TA- and ΔN isoforms.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Synthetic peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/mL (1:250 - 1:1000 dilution). For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL (1:100 - 1:250 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody does not recognize TA isoforms of p63.

Due to complete sequence homology between the immunizing peptide and mouse p63 (?N), this clone is predicted to recognize mouse p63 (?N) isoforms.

Product Citations
  1. Zhong Z, et al. 2022. Bio Protoc. 12: . PubMed
  2. Chalmers FE, et al. 2022. Dev Biol. 485:9. PubMed
RRID
AB_2820119 (BioLegend Cat. No. 699501)
AB_2820119 (BioLegend Cat. No. 699502)

Antigen Details

Structure
p53 family, six isoforms, ΔN p63α, β, γ, approximately 66 kD, 52 kD, 45 kD
Distribution

Nuclear

Function
Essential for limb formation, epidermal morphogenesis, epithelial stem cell regeneration. ΔN isoforms are antagonistic to p53.
Interaction
p53, p73, TA- and ΔN isoforms
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Tumor Suppressors
Antigen References

1. Yang A, et al. 1998. Mol Cell. 2:305. 
2. Celli J, et al. 1999. Cell. 99:143. 
3. Gaiddon C, et al. 2001. Mol Cell Biol. 21:1874. 
4. Benard J, et al. 2003. Hum Mutat. 21:182.
5. Mangiulli M, et al. 2009. Nucleic Acids Res. 37:6092

Gene ID
8626 View all products for this Gene ID
UniProt
View information about p63 on UniProt.org
Go To Top Version: 1    Revision Date: 07/19/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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