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Purified anti-PAK1 Antibody

Pricing & Availability
Clone
A17137A (See other available formats)
Regulatory Status
RUO
Other Names
PAK1, p21-activated kinase 1
Isotype
Mouse IgG2a, κ
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Product Citations
publications
A17137A_Pure_PAK1_Antibody_1_110118
Western blot of purified anti-PAK1 antibody (clone A17137A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17137A_Pure_PAK1_Antibody_1_110118
    Western blot of purified anti-PAK1 antibody (clone A17137A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17137A_Pure_PAK1_Antibody_2_110118
    IHC staining of purified anti-PAK1 antibody (clone A17137A) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • A17137A_Pure_PAK1_Antibody_3_110118
    IHC staining of purified anti-PAK1 antibody (clone A17137A) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • A17137A_Pure_PAK1_Antibody_4_110118
    IHC staining of purified anti-PAK1 antibody (clone A17137A) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
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856701 25 µg £73
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Description

PAK1 is a ubiquitously expressed protein that belongs to the group I PAK family of serine/threonine kinases, which are important mediators that link the Rho family GTPase signaling to cytoskeleton remodeling. PAK1 activation has been shown to play a role in tumorgenesis, actin dynamics, cell cycle progression, cell motility, and angiogenesis. The PAK family of kinases is affected in several brain diseases, including neurofibromatosis model of autism, Alzheimer’s Disease, Fragile X syndrome and schizophrenia1. Recently studies using electrophysiological recordings of hippocampal CA1 synaptic currents suggest that PAK1 plays a role in inhibitory synaptic transmission, where knocking out PAK affects the excitatory/inhibitory balance in the hippocampus, and may lead to dysregulation of synaptic strength.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.2 - 2.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783462 (BioLegend Cat. No. 856701)

Antigen Details

Structure
PAK1 is a 545 amino acid protein with an apparent molecular mass of ~ 61 kD.
Distribution

Tissue distribution: Ubiquitously expressed in all tissues.

Cellular distribution: Cytosol, plasma membrane, and nucleus.

Function
PAK1 plays an important role in regulating cytoskeletal dynamics, spine morphology, and synaptic plasticity.
Interaction
Forms homodimer, NISCH, DVL1, MUSK, CDC2L1, CDC2L2, p110C, CDC42/P21, RAC1, DSCAM, PDPK1, RAF1, NCK1, NCK2
Cell Type
Astrocytes, Microglia, Neurons, Oligodendrocytes
Biology Area
Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References
  1. Xia S, et al. 2018. Small GTPases. 9(4):322
  2. Kreis P, et al. 2009. Cell Signal. 21(3):384
  3. Nikolic M. 2008. Mol Neurobiol. 37(2-3): 187
  4. Salminen A, et al. 2008. Biochem Biophys Res Commun. 371(4):587
Gene ID
5058 View all products for this Gene ID 18479 View all products for this Gene ID 29431 View all products for this Gene ID
UniProt
View information about PAK1 on UniProt.org
Go To Top Version: 1    Revision Date: 11/02/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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