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Purified anti-PAK1 Antibody

Pricing & Availability
Clone
A17137B (See other available formats)
Regulatory Status
RUO
Other Names
p21-activated kinase 1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17137B_Purified_PAK1_Antibody_1_072519
Western blot of purified anti-PAK1 antibody (clone A17137B). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain lysate; Lane 3: 10 µg of mouse brain lysate; Lane 4: 10 µg of rat brain lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17137B_Purified_PAK1_Antibody_1_072519
    Western blot of purified anti-PAK1 antibody (clone A17137B). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain lysate; Lane 3: 10 µg of mouse brain lysate; Lane 4: 10 µg of rat brain lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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868601 25 µg £73
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Description

PAK1 is a ubiquitously expressed protein that belongs to the group I PAK family of serine/threonine kinases, which are important mediators that link the Rho family GTPase signaling to cytoskeleton remodeling. PAK1 activation has been shown to play a role in tumorgenesis, actin dynamics, cell cycle progression, cell motility, and angiogenesis. The PAK family of kinases is affected in several brain diseases, including neurofibromatosis model of autism, Alzheimer’s disease, Fragile X syndrome, and schizophrenia1. Recent studies using electrophysiological recordings of hippocampal CA1 synaptic currents suggest that PAK1 plays a role in inhibitory synaptic transmission, where knocking out PAK1 affects the excitatory/inhibitory balance in the hippocampus, and may lead to dysregulation of synaptic strength.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814622 (BioLegend Cat. No. 868601)

Antigen Details

Structure
PAK1 is a 545 amino acid protein with predicted and observed molecular mass of ~61 kD.
Distribution

Tissue distribution: Ubiquitously expressed in all tissues
Cellular distribution: Cytosol, plasma membrane, and nucleus

Function
PAK1 plays an important role in regulating cytoskeletal dynamics, spine morphology, and synaptic plasticity.
Interaction
PAK1 forms homodimers, and interacts with: NISCH, DVL1, MUSK, CDC2L1, CDC2L2, p110C, CDC42/P21, RAC1, DSCAM, PDPK1, RAF1, NCK1, and NCK2.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Cell Structure, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Cytoskeletal Proteins, Enzymes and Regulators, GPCR, Microtubules, Protein Kinases/Phosphatase
Antigen References
  1. Xia S, et al. 2018. Small GTPases. 9(4):322
  2. Kreis P, et al. 2009. Cell Signal. 21(3):384
  3. Nikolic M. 2008. Mol Neurobiol. 37(2-3): 187
  4. Salminen A, et al. 2008. Biochem Biophys Res Commun. 371(4):587
Gene ID
5058 View all products for this Gene ID 18479 View all products for this Gene ID 29431 View all products for this Gene ID
UniProt
View information about PAK1 on UniProt.org
Go To Top Version: 1    Revision Date: 07/25/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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