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Purified anti-Parvalbumin Antibody

Pricing & Availability
Clone
A17130G (See other available formats)
Regulatory Status
RUO
Other Names
Parvalbumin alpha (PVALB), D22S749
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A17130G_PURE_PARVALBUMIN_1-IHC-P_101923
IHC staining of purified anti-Parvalbumin (clone A17130G) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated without (panel A) and with (panel B) 5 µg/mL of purified anti-Parvalbumin (clone A17130G) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X objective. Scale bar: 50 µm
  • A17130G_PURE_PARVALBUMIN_1-IHC-P_101923
    IHC staining of purified anti-Parvalbumin (clone A17130G) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated without (panel A) and with (panel B) 5 µg/mL of purified anti-Parvalbumin (clone A17130G) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X objective. Scale bar: 50 µm
  • A17130G_PURE_PARVALBUMIN_2-IHC-P_101923
    IHC staining of purified anti-Parvalbumin (clone A17130G) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated with 5 µg/mL of purified anti-Parvalbumin (clone A17130G) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
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626151 25 µg £110
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626152 100 µg £278
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Description

Parvalbumin is a calcium-binding protein with diverse functions in various tissues. Originally identified in skeletal muscle fibers, parvalbumin holds particular significance in the central nervous system. In neurons, parvalbumin acts as a calcium buffer, helping to maintain precise control over calcium ion levels within the cell. This function is critical for regulating synaptic transmission, especially at inhibitory synapses where it influences the release of the neurotransmitter GABA to synchronize the activity of neural networks. Additionally, parvalbumin contributes to the plasticity of synaptic connections, affecting learning and memory. Overall, parvalbumin's multifaceted role underscores its importance in shaping the intricate dynamics of the nervous system and maintaining its proper functioning.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant N-terminus fragment of human parvalbumin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For IHC-P in mouse tissues, we recommend antigen retrieval with Citrate Buffer, 10X (Cat. No. 420901). 

Clone A17130G is not suitable for Western Blot.

Clone A17130G is not suitable for ICC. 

Clone A17130G is not suitable for IHC-P in human tissues. Although signal is detected, signal strength is low.

RRID
AB_3097265 (BioLegend Cat. No. 626151)
AB_3097265 (BioLegend Cat. No. 626152)

Antigen Details

Structure
Parvalbumin is a 110 amino acid protein with a predicted molecular weight of 12.1 kD
Distribution

Cytoplasmic; Inhibitory neurons, GABA-ergic neurons

Function
Calcium regulation
Cell Type
GABAergic Neurons, Mature Neurons, Neurons
Biology Area
Signal Transduction
Antigen References
  1. Permyakov EA, et al. 2017. Curr Protein Pept Sci. 18:780-794.
  2. Rupert DD and Shea SD. 2022. Front Neural Circuits. 16:886629. 
  3. Hu H, et al. 2014. Science. 345:1255263.
  4. Juarez P and Martinez Cerdeno V. 2022. Front Psychiatry. 13:913550. 
Gene ID
5816 View all products for this Gene ID
UniProt
View information about Parvalbumin on UniProt.org
Go To Top Version: 1    Revision Date: 10/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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