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Purified anti-PDCD4 Antibody

Pricing & Availability
Clone
W18083A (See other available formats)
Regulatory Status
RUO
Other Names
Programmed Cell Death 4, PDCD4, Programmed Cell Death Protein 4, PDCD-4
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18083A_PURE_PDCD4_Antibody_1_121119_updated.png
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-PDCD4 Antibody (clone W18083A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W18083A_PURE_PDCD4_Antibody_1_121119_updated.png
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-PDCD4 Antibody (clone W18083A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W18083A_PURE_PDCD4_Antibody_2_121119_updated.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400502) or purified anti-PDCD4 antibody (clone W18083A). The resulting IP fractions and whole cell extract input (5%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 1.0 µg/mL of purified anti-PDCD4 antibody (clone W18083A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP anti-rat light chain specific antibody. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W18083A_PURE_PDCD4_Antibody_3_121119.png
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1 µg/mL of either purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400502) or purified anti-PDCD4 antibody (clone W18083A, panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG antibody (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
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933501 25 µg £70
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933502 100 µg £174
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Description

Programmed cell death protein 4 (PDCD4) is a multifunctional tumor suppressor, which inhibits cell growth, metastasis and induces apoptosis by interacting with numerous factors. PDCD4 binds with eukaryotic initiation factors EIFAI and EIFAII, inhibiting their RNA helicase activity and cap-dependent translation, thereby impeding protein synthesis.

PDCD4 localizes to the nuleoplasm of cells.

Diseases linked to PDCD4 include Bardet-Biedl syndrome and colorectal cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human PDCD4 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1 µg/mL. For immunocytochemistry, a concentration range of 1 - 5 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone recognizes human and mouse PDCD4.

For ICC testing, HeLa cells were fixed in 4% PFA and permeabilized with either Triton X-100 or methanol; both methods produced strong staining at 1 mg/mL.

RRID
AB_2820207 (BioLegend Cat. No. 933501)
AB_2820207 (BioLegend Cat. No. 933502)

Antigen Details

Structure
PDCD4 is a 469 amino acid protein with predicted molecular weight of 52 kD. PDCD4 contains MI domains and a nuclear localization signal.
Distribution

PDCD4 localizes to the nuleoplasm of cells / Ubiquitously expressed.

Function
Multifunctional tumor suppressor
Interaction
RNA binding, EIF4A1 and EIF4G
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Death, Cell Proliferation and Viability
Molecular Family
Protein Modifiers, Tumor Suppressors
Antigen References
  1. Matsuhashi S, et al. 2019. Int. J. Mol. Sci. 20(9): 2304.
  2. Cmarik JL, et al. 1999. Proc. Natl. Acad. Sci. U S A. 96(24):14037-42
  3. Suzuki C, et al. 2008. Proc. Natl. Acad. Sci. U S A. 105(9):3274-9
  4. Yuam H, et al. 2017. Oncol. Lett. 14(3):3371-8
  5. Yang HS, et al,  2003. Mol Cell Biol 23 (1):26-37
Gene ID
27250 View all products for this Gene ID
UniProt
View information about PDCD4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/11/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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