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Purified anti-Phostensin Antibody

Pricing & Availability
Clone
4B12-E11-D9 (See other available formats)
Regulatory Status
RUO
Other Names
Protein Phosphatase 1 Regulatory Subunit 18, Protein Phosphatase 1 F-Actin Cytoskeleton Targeting Subunit, KIAA1949
Isotype
Mouse IgG1, κ
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Product Citations
publications
Western blot of purified anti-Phostensin antibody (4B12-E11-D9). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain cytoskeleton lysate. The blot was incubated with 2.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled Goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • Western blot of purified anti-Phostensin antibody (4B12-E11-D9). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain cytoskeleton lysate. The blot was incubated with 2.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled Goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • IHC staining of purified anti-Phostensin antibody (4B12-E11-D9) on formalin-fixed paraffin-embedded human lymph node tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2.0 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
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865801 25 µg £73
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865802 100 µg £189
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Description

Phostensin is a protein that targets protein phosphatase 1 (PP1) to F-actin cytoskeleton. The phostensin/PP1 complex is involved in actin cytoskeleton remodeling. Diseases associated with phostensin include deafness and  autosomal recessive 79.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant human phostensin protein fragments expressed in E.coli
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810781 (BioLegend Cat. No. 865801)
AB_2810781 (BioLegend Cat. No. 865802)

Antigen Details

Structure
Phostensin is a 613 amino acid protein with an observed molecular mass of ~100 kD.
Distribution

Tissue Distribution: Brain, spleen, lymph node, and leukocytes
Cellular Distribution: Cytoskeleton, nucleus, and cytosol

Function
Phostensin is involved in the regulation of protein phosphase-1 activity by targeting it to the F-actin cytoskeleton.
Interaction
Protein Phosphatase-1, F-actin
Cell Type
Lymphocytes, Neurons
Biology Area
Cell Biology, Immunology, Neurodegeneration, Neuroscience, Signal Transduction
Molecular Family
Cytoskeletal Proteins, Enzymes and Regulators, Phospho-Proteins, Protein Modifiers
Antigen References
  1. Matsubara T, et al. 2018. Mol Cell Biol. 38(4). pii: e00425.
  2. Lin YS, et al. 2011. J Histochem Cytochem. 59(8):741.
Gene ID
170954 View all products for this Gene ID
UniProt
View information about Phostensin on UniProt.org
Go To Top Version: 1    Revision Date: 05/09/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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