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Purified anti-phycoerythrin (PE) (Maxpar® Ready) Antibody

Pricing & Availability
Clone
PE001 (See other available formats)
Regulatory Status
RUO
Other Names
PE
Isotype
Mouse IgG1, κ
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Product Citations
publications
PE001_Purified_PE_Cytof_061014.jpg
Human PBMC stained with PE-anti-CD3 (UCHT1) then detected with 145Nd-anti-PE (PE001). Plotted against 154Sm-anti-CD45 (HI30). Data provided by DVS Sciences.
  • PE001_Purified_PE_Cytof_061014.jpg
    Human PBMC stained with PE-anti-CD3 (UCHT1) then detected with 145Nd-anti-PE (PE001). Plotted against 154Sm-anti-CD45 (HI30). Data provided by DVS Sciences.
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408105 100 µg £97
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Description

The PE001 antibody reacts with both phycoerythrin (PE) alone and PE conjugated with antibody or streptavidin, as well as PE tandem dyes such as PE/Cyanine5, PE/Cyanine5.5 and PE/Cyanine7. When PE001 antibody is used in a three-step staining procedure or separation of PE-antibody labeled cells, it does not quench PE fluorescence or react with tested mouse and human cells. The PE001 antibody can be used for separation of PE-antibody labeled cells or as a secondary step to amplify PE conjugated primary antibody signals (i.e. biotinylated PE001 as secondary step, followed by Streptavidin-PE).

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Phycoerythrin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C
Application

FC - Quality tested
CyTOF® - Verified
Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References

(PubMed link indicates BioLegend citation)
  1. Taylor RT, et al. 2007. J. Immunol. 178:5659.
Product Citations
  1. Gide TN, et al. 2019. Cancer Cell. 35:238. PubMed
  2. Singh RP, et al. 2018. Stem Cell Reports. 28:29. PubMed
  3. Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
RRID
AB_2563787 (BioLegend Cat. No. 408105)

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Go To Top Version: 3    Revision Date: 09/16/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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