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Purified anti-PINK1 Antibody

Pricing & Availability
Clone
DU46-1.1 (See other available formats)
Regulatory Status
RUO
Other Names
PTEN induced putative kinase 1, BRPK, PARK6, Serine/threonine-protein kinase PINK1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
DU46-11_PURE_PINK1_Antibody_1_101519
Western blot of purified anti-PINK1 antibody (clone DU46-1.1). Lane 1: Molecular weight marker; Lane 2: 20 µg of control HeLa cell lysate; Lane 3: 20 µg of CCCP-treated HeLa cell lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • DU46-11_PURE_PINK1_Antibody_1_101519
    Western blot of purified anti-PINK1 antibody (clone DU46-1.1). Lane 1: Molecular weight marker; Lane 2: 20 µg of control HeLa cell lysate; Lane 3: 20 µg of CCCP-treated HeLa cell lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • DU46-11_PURE_PINK1_Antibody_2_101519
    ICC staining of purified anti-PINK1 antibody (clone DU46-1.1) on control (left) or CCCP (right) treated HeLa cells. The cells were treated with 10 µM of CCCP for 24 hours. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 5 μg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for one hour at room temperature. The cells were counterstained with Flash Phalloidin™ Green 488 (Cat. No. 424201) at a 1:100 dilution for 20min at room temperature. The slides were mounted with fluoromount G with DAPI. The images were captured with a 60X objective. Scale bar: 20 µm
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846201 25 µg £73
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846202 100 µg £182
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Description

PINK1 (PTEN-induced putative kinase 1) is a 63 kD mitochondrial transmembrane protein that is often cleaved by PARL into a 53 kD fragment. The protein is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). PINK1 phosphorylation of mitochondrial proteins can protect against mitochondrial dysfunction due to cellular stresses. PINK1 mutations can cause autosomal recessive early-onset Parkinson disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Yao J, et al. 2022. Autophagy. 18:1879. PubMed
  2. Lin Q, et al. 2020. JCI Insight. :5. PubMed
  3. Fiesel FC, et al. 2023. Autophagy. 1711:19. PubMed
  4. Bharath LP, et al. 2020. Cell Metabolism. 32(1):44-55.e6.. PubMed
  5. Chung E, et al. 2020. Sci Adv. 6:eaba1193. PubMed
RRID
AB_2572127 (BioLegend Cat. No. 846201)
AB_2572127 (BioLegend Cat. No. 846202)

Antigen Details

Structure
PINK1 is a 581 amino acid, 63 kD, transmembrane protein that contains an N-terminal mitochondrial localization sequence, a serine/threonine kinase domain, and a C-terminal regulatory domain. PINK1 is proteolytically cleaved by PARL into a 55 kD fragment.
Distribution

Mitochondria and cytoplasm.

Function
PINK1 is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of Parkin protein (PARK2). PARL-cleaved PINK1 is a cytoplasmic probe for damaged mitochondria. PINK1 phosphorylation of mitochondrial proteins can protect against mitochondrial dysfunction due to cellular stresses.
Interaction
PARKIN, Beclin 1, FBX07, DJ-1, HSP90/CDC37.
Ligand/Receptor
PARKIN, Beclin 1, FBX07, DJ-1, HSP90/CDC37.
Biology Area
Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family
Mitochondrial Markers
Antigen References

1. Trempe JF, Fon EA. 2013. Front Neurol. 4:38.
2. Durcan TM, Fon EA. 2015. Genes Dev. 29(10):989.

Gene ID
65018 View all products for this Gene ID
UniProt
View information about PINK1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10/15/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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