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Purified anti-Polyubiquitin (K63-linkage) Antibody

Pricing & Availability
Clone
HWA4C4 (See other available formats)
Regulatory Status
RUO
Other Names
Polyubiquitin-C, Polyubiquitin-C precursor, HMG20, Ub-K63, UbK63
Isotype
Mouse IgG2a, λ
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Product Citations
publications
HWA4C4_PURE_Polyubiquitin_Antibody_1_101019
Western blot of purified anti-Polyubiquitin (K63-linkage) antibody (clone HWA4C4). Lane 1: Molecular weight marker; Lane 2: 30 µg of HepG2 cell lysate; Lane 3: 30 µg of NIH 3T3 cell lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • HWA4C4_PURE_Polyubiquitin_Antibody_1_101019
    Western blot of purified anti-Polyubiquitin (K63-linkage) antibody (clone HWA4C4). Lane 1: Molecular weight marker; Lane 2: 30 µg of HepG2 cell lysate; Lane 3: 30 µg of NIH 3T3 cell lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • HWA4C4_PURE_Polyubiquitin_Antibody_2_101019
    Western blot of purified anti-Polyubiquitin (K63-linkage) antibody (clone HWA4C4). Lane 1: Molecular weight marker; Lane 2: 1 µg of Human Poly-Ub WT Chains (1-7) K63 linked protein. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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932201 25 µg £70
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932202 100 µg £197
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Description

Polymeric or monomeric Ubiquitin exists free (unanchored) or covalently attached to its ligand. Ubiquitin is conjugated to its target via an isopeptide bond either as a monomer or as one of two polymeric forms. Ubiquitin monomers in one type of polymer are covalently linked via various Lys residues (polyubiquitin). The other type of ubiquitin polymer involves monomers that are linked linearly via an initiator Met (linear polyubiquitin). Polyubiquitin’s functions depend on which Lys residue is involved in cross-linking the protein. K63-linked polyubiquitin chains exhibit nonproteolytic function in vivo which includes kinase/phosphatase activation, protein trafficking, endocytosis, DNA-damage responses, as well as in signaling processes which lead to the activation of the transcription factor NF-κ-B.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide containing the ubiquitin K-63 isopeptide motif.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-F, ICC, IP - Reported in the literature, not verified in house, not verified in-house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone HWA4C4 detects Polyubiquitin chains formed by the linkage with Lys63 residue.

It does not cross-react with any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitinylated species.

Application References

(PubMed link indicates BioLegend citation)
  1. Dammer E, et al. 2011. J. Biol. Chem. 286:10457. (IHC-F, ICC, WB)
  2. Lin S, et al. 2013. J. Virology 87:11562. (ICC, IP, WB)
  3. Karlsson AB, et al. 2014. Mol. Biol. Cell 25:1355. (WB, ICC)
RRID
AB_2566131 (BioLegend Cat. No. 932201)
AB_2566131 (BioLegend Cat. No. 932202)

Antigen Details

Structure
685 amino acids; 77 kD.
Distribution

Cytoplasmic, nuclear, and extracellular.

Function
Polyubiquitin-C is involved in Poly(A) RNA, protein, and protease binding. The polymeric protein is involved in protein degradation, DNA repair, kinase modification, endocytosis, and cell cycle regulation.
Interaction
Numerous targets including CDK-mediated phosphorylation and removal of CDc6, loss of function of SMAD2/3 in cancer, and signaling by NOTCH1 PEST Domain Mutants in cancer.
Ligand/Receptor
Poly(A) RNA, protein, and proteases.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance, Signal Transduction
Antigen References

1. Radici L, et al. 2013. Advances in Bioscience and Biotechnology. 4:1057.
2. Walczak H, et al. 2012. Generation and physiological roles of linear ubiquitin chains. BMC Biol. 10:23.

Gene ID
7316 View all products for this Gene ID
UniProt
View information about Polyubiquitin on UniProt.org
Go To Top Version: 2    Revision Date: 10/11/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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