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Purified anti-PPM1B Antibody

Pricing & Availability
Clone
W19264A (See other available formats)
Regulatory Status
RUO
Other Names
Protein Phosphatase 1B, PP2CB, PP2C-beta, PP2C-beta-X, Protein Phosphatase, Mg2+/Mn2+ Dependent 1B, Ser/Thr Protein Phosphatase Type 2C Beta 2 Isoform
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W19264A_PURE_PPM1B_Antibody_1_081921.png
Whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-PPM1B antibody (clone W19264A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W19264A_PURE_PPM1B_Antibody_1_081921.png
    Whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-PPM1B antibody (clone W19264A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W19264A_PURE_PPM1B_Antibody_2_081921.png
    Whole cell extracts (15 µg total protein) from HeLa cells treated with non-targeting control siRNA (siCON) or siRNA targeting PPM1B (siPPM1B) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-PPM1B antibody (clone W19264A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
Cat # Size Price Quantity Check Availability Save
934201 25 µg £81
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934202 100 µg £201
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Description

Protein phosphatase 1B (PPM1B) is a member of the Protein Phosphatase 2C (PP2C) family of monomeric metal-dependent Ser/Thr protein phosphatases which are dependent on Mg2+ and Mn2+ for their catalytic activity, and function in the negative regulation of cellular stress signaling pathways. PPM1B contributes to the regulation of both cell senescence and proliferation as a regulator of the p38 MAPK pathway and negatively regulates necroptosis through dephosphorylation of receptor-interacting protein 2 (RIP3), one of the primary mediators of necrotic programmed cell death. It is one of a limited number of proteins that can dephosphorylate nuclear receptor PPARγ, thereby stimulating its transcription and contributing to its role in cellular differentiation and metabolism. It is also associated with the dephosphorylation of p53, TAK1, and, in some cell types, the negative regulation of NF-κB signaling through dephosphorylation of nuclear factor kappa B kinase subunit β (IKKβ). PPM1B plays a role in the mediation of antiviral response by acting as a TBK1 phosphatase via dephosphorylation at Ser172 and suppresses cancer cell proliferation and tumor progression through the dephosphorylation of Rho guanine nucleotide dissociation inhibitor 1 (RhoGDI1), a key regulator of Rho GTPases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human PPM1B protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality testedKO/KD-WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone failed to stain PPM1B in HeLa cells fixed with 4% PFA and permeabilized with either Triton X-100 or methanol. It also failed to stain cells fixed and permeabilized with methanol.

Additional Product Notes

This clone failed to stain PPM1B in HeLa cells fixed with 4% PFA and permeabilized with either Triton X-100 or methanol. It also failed to stain cells fixed and permeabilized with methanol.

RRID
AB_2894521 (BioLegend Cat. No. 934201)
AB_2894521 (BioLegend Cat. No. 934202)

Antigen Details

Structure
PPM1B is 479 amino acid protein with a predicted molecular weight of 52 kD.
Distribution

Ubiquitously expressed/ cytosol and membrane

Function
Protein phosphatase
Biology Area
Cell Biology
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Cho H, et al. 2018. Cancer Letters. 417:141
  2. Park J, et al. 2014. Mech Ageing Dev. 138:45.
  3. Tasdelen I, et al. 2013. Biochem J. 451:45.
  4. Wanz C, et al2015. Nat Cell Biol. 17:434.
  5. Zhao Y, et al. 2012. Cell Signal. 24:2197.
Gene ID
5495 View all products for this Gene ID
UniProt
View information about PPM1B on UniProt.org
Go To Top Version: 1    Revision Date: 08/19/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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