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Purified anti-Peroxiredoxin 1 Antibody

Pricing & Availability
Clone
6A6-4H3-7A3 (See other available formats)
Regulatory Status
RUO
Other Names
PRDX1, Natural killer cell-enhancing factor A, NKEF-A, Proliferation-associated gene protein, PAG, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2, Thioredoxin-dependent peroxiredoxin 1, PAGA, PAGB, TDPX2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
6A6-4H3-7A3_PURE_PRDX1_Antibody_1_021723
Whole cell extracts (15 µg total protein) of the indicated cell lines were resolved by a 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-Peroxiredoxin 1 (clone 6A6-4H3-7A3) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • 6A6-4H3-7A3_PURE_PRDX1_Antibody_1_021723
    Whole cell extracts (15 µg total protein) of the indicated cell lines were resolved by a 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-Peroxiredoxin 1 (clone 6A6-4H3-7A3) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • 6A6-4H3-7A3_PURE_PRDX1_Antibody_2_020623
    ICC staining of purified anti- Peroxiredoxin 1 (clone 6A6-4H3-7A3) on HeLa cells. Cells were fixed and permeabilized with ice-cold methanol, blocked with 5% FBS for 1 hour at room temperature, then were stained with either 5 µg/mL of purified mouse IgG1, κ isotype control (Cat. No. 400102) (panel A) or 5 µg/mL of anti-Peroxiredoxin 1 (panel B) overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801), and the images were captured with a 63X objective. Scale bar: 20 µm
Cat # Size Price Quantity Check Availability Save
611151 25 µg £83
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611152 100 µg £260
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Description

Peroxiredoxin 1, also called PRDX1, is one of six members of the superfamily of thiol-dependent peroxidases. Peroxiredoxin 1 can eliminate reactive oxygen and nitrogen species. As a critical antioxidant enzyme, it utilizes an N-terminal cystine to detoxify peroxides and protect cells from oxidative stress damage. As a sensor of inflammation response, extracellular Peroxiredoxin 1 is released to trigger TLR4-dependent secretion of TNF-α and IL-6 in macrophage and dendritic cell maturation. Peroxiredoxin 1 is increased in bone marrow-derived macrophages by lipopolysaccharide (LPS) and IFN-γ exposure. In microglia, the expression level of Peroxiredoxin 1 is regulated through ROS/p38/MAPK pathway and protects microglia from cell death. Overexpression of Peroxiredoxin 1 in rat primary hippocampus neuronal and PC12 cells promotes survival under amyloid β toxicity. In addition to its role as an antioxidant, oxidized Peroxiredoxin 1 forms an oligomer that acts as a chaperone to suppress the c-Myc oncogene and promotes tumor cell apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein of human Peroxiredoxin 1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration of 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product is not recommended for use in IHC-P.

For immunocytochemistry, fixation with ice-cold methanol is recommended.

RRID
AB_2936735 (BioLegend Cat. No. 611151)
AB_2936735 (BioLegend Cat. No. 611152)

Antigen Details

Structure
Peroxiredoxin 1 is a 199 amino acid protein with an expected molecular weight of 22 kD. It exists as a homodimer and homodecamer.
Distribution

Mainly expressed in cytoplasm; Distributed ubiquitously across tissues

Function
Catalyzes the reduction of hydrogen peroxide and organic hydroperoxides
Interaction
Peroxiredoxin 1, PTEN, Androgen receptor, C-ABL
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Signal Transduction, Transcription Factors
Antigen References
  1. Shau H, et al. 1993. Cell Immunol. 147:1-11.
  2. Wood ZA, et al. 2003. Trends Biochem. Sci. 28:32-40.
  3. Cumming RC, et al. 2007. J Biol Chem. 282:30523-34.
  4. Kim SU, et al. 2008. Biol Pharm Bull. 31:820-5.
Gene ID
5052 View all products for this Gene ID
UniProt
View information about Peroxiredoxin 1 on UniProt.org
Go To Top Version: 1    Revision Date: 02/06/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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