- Clone
- A18224E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Profilin 1, PFN1, Profilin1, Epididymis tissue protein Li 184a
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
631751 | 25 µg | £117 | ||||
631752 | 100 µg | £278 |
Profilin-1 is an actin-binding protein responsible for the regulation of actin dynamics. Profilin-1 assists in the exchange of ADP for ATP on G-actin, facilitating the polymerization of actin monomers into filaments by promoting assembly at the growing end. Thus, profilin-1 is implicated in processes like cell motility, cytokinesis, and endocytosis, highlighting its significance in maintaining cellular structure and function. Profilin-1 also interacts with numerous proteins involved in signaling pathways, influencing cellular responses to intracellular and extracellular cues. Emerging evidence also shows that profilin-1 can bind to microtubules, facilitating interactions between the two major components of the cytoskeleton. Mutations in profilin-1 have been associated with amyotrophic lateral sclerosis (ALS) and is suspected to cause insoluble aggregates due to disruption in axonal outgrowth, cell integrity and protein distribution.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant fragment of the N terminus of human Profilin 1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody (clone A18224E) is not suitable for immunocytochemistry (ICC).
For immunohistochemistry (IHC), we recommend antigen retrieval with Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0). Antigen retrieval with Sodium Citrate is not recommended as it results in mis-localized signal. - RRID
-
AB_3097566 (BioLegend Cat. No. 631751)
AB_3097566 (BioLegend Cat. No. 631752)
Antigen Details
- Structure
- Profilin-1 is an 140 amino-acid protein with a predicted molecular weight of 15 kD.
- Distribution
-
Ubiquitous, cytoskeleton/cytoplasm
- Function
- Actin dynamics, cell shape and motility
- Interaction
- Actin, Ena/VASP proteins, phosphoinositides, ROCK, RAP1, CDC42
- Ligand/Receptor
- Profilin-1 binds actin monomers
- Biology Area
- Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure
- Molecular Family
- Microfilaments
- Antigen References
-
- Murk K, et al. 2021. Front Cell Dev Biol. 9:681122.
- Karlsson R, et al. 2021. J Cell Physiol. 236:7256-7265.
- Davey RJ, et al. 2020. Biophys Rev. 12:827-849.
- Gene ID
- 5216 View all products for this Gene ID
- UniProt
- View information about Profilin-1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Profilin-1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Profilin-1 | A18224E | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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