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Purified anti-PSMA6 Antibody

Pricing & Availability
Clone
O91F3 (See other available formats)
Regulatory Status
RUO
Other Names
PSMA6, proteasome subunit alpha type-6, PSA6, 27 kDa prosomal protein, PROS-27, p27K, multicatalytic endopeptidase complex iota chain, proteasome iota chain
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
O91F3_PURE_PSMA6_Antibody_081219
Whole cell extracts (15 µg protein) from the indicated cell lines, HEK-293, HeLa, BeWo, HepG2, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-PSMA6 antibody, clone O91F3, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker.
  • O91F3_PURE_PSMA6_Antibody_081219
    Whole cell extracts (15 µg protein) from the indicated cell lines, HEK-293, HeLa, BeWo, HepG2, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-PSMA6 antibody, clone O91F3, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker.
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670601 25 µg £70
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670602 100 µg £174
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Description

Proteasome subunit-α type-6 protein, or PSMA6, is an essential subunit of the 20S catalytic core proteasome complex. As a component of the complex, it plays a central role in the proteolytic degradation of ubiquitinated proteins, thereby, maintaining protein homeostasis by removing misfolded, damaged, or excess proteins. It is involved in multiple pathways including spermatogenesis and peptide processing for MHC class I binding, and has been proposed as a therapeutic target for lung cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length recombinant human PSMA6 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

O91F3 failed ICC testing; HeLa cells were fixed with 4% paraformaldehyde and tested using two permeabilization methods (Methanol and Triton X-100). The clone failed ICC testing due to non-specific staining of cytosol.

 

IP has not been tested on clone O91F3.

RRID
AB_2814522 (BioLegend Cat. No. 670601)
AB_2814522 (BioLegend Cat. No. 670602)

Antigen Details

Structure
PSMA6 is a 246 amino acid protein with a predicted molecular weight of 27 kD.
Distribution

Nucleus/Ubiquitously expressed

Function
Protein homeostasis, proteostasis
Biology Area
Ubiquitin/Protein Degradation
Antigen References
  1. Li Z, et al. 2010. Biochem. Cell. Biol. 88:635-48.
  2. Wenzel T, Baumeister W. 1995. Nat. Struct. Biol. 2:199-204. 
Gene ID
5687 View all products for this Gene ID
UniProt
View information about PSMA6 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/02/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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