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Purified anti-PTBP1 Antibody

Pricing & Availability
Clone
3H7 (See other available formats)
Regulatory Status
RUO
Other Names
Polypyrimidine tract binding protein 1, Heterogeneous nuclear ribonucleoprotein I, hnRNP I, 57 kD RNA-binding protein PPTB-1, PTB
Isotype
Mouse IgG1, κ
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Product Citations
publications
3H7_IHC_062508
Formalin-fixed paraffin-embedded human kidney tissue was stained with 3H7 at 15 µg/ml and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
  • 3H7_IHC_062508
    Formalin-fixed paraffin-embedded human kidney tissue was stained with 3H7 at 15 µg/ml and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
  • PTBP1_012307
    Extracts from human PBMC were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal antibody against PTBP1 (clone 3H7). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
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630101 25 µg £81
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Description

The 3H7 monoclonal antibody recognizes human PTBP1 also known as Polypyrimidine tract binding protein 1, Heterogeneous nuclear ribonucleoprotein I, and 57 kD RNA-binding protein. PTBP1 is a ubiquitously expressed nuclear protein with a predicted molecular weight approximately 59.6 kD. The polypyrimidine tract binding protein (PTB) is an important regulator of alternative splicing that also affects mRNA localization, stabilization, polyadenylation, and translation. PTBP1 has been shown to interact with FUS, Caspase3, PKCa, Raver1, and HnRNP-L protein. The PTB gene contains 15 exons and spans 13.5 kb. The 3H7 antibody has been shown to be useful for Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human PTBP1 (full length)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5ml antibody dilution buffer for each mini-gel. For IHC, use a 15 µg/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Gabai VL, et al. 2012. Moll Cell Biol. 32:929. PubMed.
Product Citations
  1. Gabai V, et al. 2012. Mol Cell Biol. 32:929. PubMed
  2. Pablos I, et al. 2021. Cell Rep. 37:109892. PubMed
RRID
AB_2171285 (BioLegend Cat. No. 630101)

Antigen Details

Structure
PTBP1 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). PTBP1 contains four RNA recognition motifs (RRMs). Nuclear protein, predicted molecular weight approximately 59.6 kD
Distribution
Nucleus, ubiquitously expressed
Function
Plays a role in pre-mRNA splicing. Binds to the polypyrimidine tract of introns. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
Interaction
FUS, Caspase3, PKCa, Raver1, and HnRNP-L
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. GilA, et al. 1991. Genes Dev. 5 (7):1224.
2. Ghetti A, et al. 1992. Acids Res. 20:3671.
3. Romanelli MG, et al. 2000. Gene 255:267.

Gene ID
5725 View all products for this Gene ID
UniProt
View information about PTBP1 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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