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Purified anti-PYK2 Antibody

Pricing & Availability
Clone
P85E2D5 (See other available formats)
Regulatory Status
RUO
Other Names
Protein-tyrosine kinase 2-beta, Calcium-dependent tyrosine kinase, FAK2, PTK2B
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
P85E2D5_PURE_PYK2_Antibody_WB_072518
Total cell lysates (15 µg protein) from Daudi (lane 1), HeLa (lane 2) and NIH3T3 (lane 3) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose and probed with 1 µg/mL of purified anti-PYK2 antibody, clone P85E2D5 (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-PYK2 antibody or 1:10000 diluted Direct-Blot HRP anti-β-Actin antibody, clone W16197A (lower panel). Lane M: Molecular weight ladder
  • P85E2D5_PURE_PYK2_Antibody_WB_072518
    Total cell lysates (15 µg protein) from Daudi (lane 1), HeLa (lane 2) and NIH3T3 (lane 3) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose and probed with 1 µg/mL of purified anti-PYK2 antibody, clone P85E2D5 (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-PYK2 antibody or 1:10000 diluted Direct-Blot HRP anti-β-Actin antibody, clone W16197A (lower panel). Lane M: Molecular weight ladder
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685002 100 µg £182
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Description

PYK2, also known as FAK2, is a non-receptor protein kinase. It is structurally similar to FAK, with 48% identity of amino acids, 60% identity of sequences in the central kinase domain, and identical positions of 4 phosphorylation sites. FAK is expressed ubiquitously, indispensable for embryogenesis, and colocalized at focal contacts with integrins and growth factor receptors. Expression of PYK2 is restricted to the endothelium, central nervous system, and hematopoietic lineages. It is dispensable for organ development, localized throughout the cytoplasm and sensitive to intracellular Calcium signaling and G protein–coupled receptors. PYK2 is activated by multiple cytokines, growth factors, hormones, and neuropeptides. PYK2 regulates different signal transduction cascades that control cell proliferation, adhesion, migration and invasion. PYK2-deficient B cells and macrophages exhibit impaired mobility and responsiveness to chemokines.

PYK2 was shown to play pro-oncogenic roles in the progression and invasion of several human cancers, including glioblastoma, hepatocytoma, non-small-cell lung carcinoma, prostate cancer, breast cancer, multiple myeloma, and more. Inhibition of PYK2 reduced activation of Wnt/b-catenin signaling by destabilizing b-catenin, leading to downregulation of c-Myc and Cyclin D1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant fragment of PYK2 (aa 815-997) expressed in E. Coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2572201 (BioLegend Cat. No. 685002)

Antigen Details

Structure
Major isoform has 1,009 amino acids and a predicted molecular weight of 116 kD.
Distribution

Cytoplasm and cell membrane.

Function
It regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading, and bone remodeling.
Interaction
SIRPA, SH2D3C, ARHGAP10, NPHP1, ASAP1, ASAP2, ARHGAP26, SKAP2 and TGFB1I1.
Cell Type
B cells
Biology Area
Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Immunology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Adhesion Molecules, Postsynaptic proteins
Antigen References

1. Dikic I, et al. 1996. Nature 383:547.
2. Mitra SK, et al. 2005. Nat. Rev. Mol. Cell Biol. 6:56.
3. Okigaki M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:10740.
4. Schaller MD. 2010. J. Cell Sci. 123:1007.
5. Zhang Y, et al. 2014. Blood. 124:2675.
6. Dios-Esponera A, et al. 2015. Mol. Biol. Cell 26:3215.
7. Selitrennik M, et al. 2015. Oncotarget. 6:22214.
8. Zhao M, et al. 2016. PLoS One 11:e0149231.

Gene ID
2185 View all products for this Gene ID
UniProt
View information about PYK2 on UniProt.org
Go To Top Version: 3    Revision Date: 07/25/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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