Purified anti-Rab6 Antibody

Pricing & Availability
Clone
A17164B (See other available formats)
Regulatory Status
RUO
Other Names
Rab6A, Rab6B, Rab6
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17164B_PURE_Rab6_Antibody_1_100819
Western blot of purified anti-Rab6 antibody (clone A17164B). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17164B_PURE_Rab6_Antibody_1_100819
    Western blot of purified anti-Rab6 antibody (clone A17164B). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17164B_PURE_Rab6_Antibody_2_100819
    Western blot of purified anti-Rab6 antibody (clone A17164B). Lane 1: Molecular weight marker; Lane 2: 30 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Check Availability Save
868901 25 µg £73
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868902 100 µg £189
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Description

Rab proteins are small GTPases that are critical for the Golgi apparatus organization. Rab6 is the most abundant Golgi-associated Rab and is expressed in several isoforms, including Rab6aA, Rab6A’, Rab6B, and Rab6C. Rab6A and Rab6A’ are ubiquitously expressed while Rab6B is selectively expressed in neuronal cells. Rab6 isoforms are involved in intra-, early, and post-Golgi trafficking. Rab6 depletion leads to increased Golgi cisternal number and accumulation of vesicles in HeLa cells, suggesting that Rab6 affects the Golgi ribbon organization via regulation of vesicle transport.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Rab6A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The antibody also detects mouse Rab6 A & B. In mouse brain lysates, besides band corresponding to Rab6, it also detects a band at ~50kD that was stronger than the isotype control.

RRID
AB_2820174 (BioLegend Cat. No. 868901)
AB_2820174 (BioLegend Cat. No. 868902)

Antigen Details

Structure
RAB6 is a 208 amino acid protein with predicted and observed molecular mass of ~ 25 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Cytosol, Golgi apparatus, plasma membrane, and endoplasmic reticulum

Function
Rab6 plays a role in retrograde protein transportation from the plasma membrane to the Golgi apparatus, and the endoplasmic reticulum.
Interaction
RAB6IP1, RAB6KIFL, BICD1, BICD2, TMF1, DYNLRB1
Cell Type
Neurons
Biology Area
Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Synaptic Biology
Molecular Family
Golgi Markers, Organelle Markers, Postsynaptic proteins, Presynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Liu S, et al. 2015. Int Rev Cell Mol Biol. 315:1
  2. Heffernan LF, et al. 2014. Biochem Soc Trans. 42(5):1453
  3. Fernandes H, et al. 2009. Biochem Soc Trans. 37:1037
Gene ID
5870 View all products for this Gene ID 51560 View all products for this Gene ID 84379 View all products for this Gene ID 363123 View all products for this Gene ID
UniProt
View information about Rab4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/08/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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