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Purified anti-rat CD106 Antibody

Pricing & Availability
Clone
MR106 (See other available formats)
Regulatory Status
RUO
Other Names
VCAM-1, INCAM-110
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MR106_PE
Lou rat bone marrow cells stained with MR106 PE
  • MR106_PE
    Lou rat bone marrow cells stained with MR106 PE
Cat # Size Price Quantity Check Availability Save
200401 50 µg £53
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200402 500 µg £180
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Description

CD106 is a 110 kD transmembrane protein also known as VCAM-1 and INCAM-110. It is an immunoglobulin superfamily member and is expressed on bone marrow stromal cells, myeloid cells, splenic dendritic cells and endothelial cells (activated by cytokines). CD106 is a counter-receptor for VLA-4 (α4β1 integrin) and LPAM-1 (α4β7 integrin). The interactions of CD106 and VLA-4 or LPAM-1 play an important role in binding of leukocytes to activated endothelial cells and leukocyte extravasation at inflammatory sites.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat VCAM-1 transfected L5178Y cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Bevilacqua, M.P. 1993. Annu. Rev. Immunol. 11:767. (IHC)
  2. Wingerd, K.L., et al. 2002. J. Neuroscience 22:10772. (IHC)
  3. Ogawa, T., et al. 2004. Am J. Physiol. Gastrointest. Liver Physiol. 286:G702. (IHC)
Product Citations
  1. Amante F, et al. 2010. J Immunol. 185:3632. PubMed
  2. Brooks M, et al. 2017. PLoS One. doi: 10.1371/journal.pone.0169976. PubMed
RRID
AB_2214211 (BioLegend Cat. No. 200401)
AB_2214211 (BioLegend Cat. No. 200402)

Antigen Details

Structure
Ig superfamily, 110 kD
Distribution

Bone marrow stromal cells, myeloid cells, splenic dendritic cells, induced on endothelial cells

Function
Adhesion, leukocyte extravasation at sites of inflammation
Ligand/Receptor
VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin)
Cell Type
Dendritic cells, Endothelial cells
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay, A.N., et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Kinashi, T., et al. 1995. J. Leuk. Biol. 57:168.
3. Bevilacquea, M.P., 1993. Ann. Rev. Immunol. 11:767.
4. Koni, P.A., et al. 2001. J. Exp. Med. 193:741.

Gene ID
22329 View all products for this Gene ID
UniProt
View information about CD106 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/06/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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