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Purified anti-rat CD28 Antibody

Pricing & Availability
Clone
JJ319 (See other available formats)
Regulatory Status
RUO
Other Names
Tp44, T44
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
JJ319_Pure_021308
LOU rat splenocytes stained with purified JJ319, followed by anti-mouse IgG FITC
  • JJ319_Pure_021308
    LOU rat splenocytes stained with purified JJ319, followed by anti-mouse IgG FITC
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200902 500 µg £174
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Description

CD28 is a 44 kD glycoprotein also known as Tp44 or T44. It is a member of the immunoglobulin superfamily and is expressed on thymocytes, α/βTCR+ T cells, most γ/δTCR+ T cells, and an NK cell subset. During interaction with CD80 (B7-1) or CD86 (B7-2), CD28 acts as the second signal for T cell stimulation. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat CD28-transfected BALB/c mouse A2OJ cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The JJ319 antibody has been reported to augment in vitro T cell proliferation and enhance IL-2 production. Additional reported applications (for the relevant formats) include: immunoprecipitation, in vitro costimulation of proliferation responses1, and in vitro costimulation of IL-2 production in the presence of R73 antibody (anti-rat a/ß TCR, Cat. No. 201112)2.

Application References

(PubMed link indicates BioLegend citation)
  1. Tacke M, et al. 1995. J. Immunol. 154:5121. (Costim)
  2. Tacke M, et al. 1997. Eur. J. Immunol. 27:239. (Costim)
  3. Du Y, et al. 2012. PLoS One. 7:e52911. PubMed.
Product Citations
  1. Kumar RK, et al. 2021. Vascul Pharmacol. 138:106838. PubMed
  2. Du Y, et al. 2012. PLoS One. 7:e52911. PubMed
  3. Reese SR, et al. 2021. Transplantation. 105:1516. PubMed
RRID
AB_313891 (BioLegend Cat. No. 200902)

Antigen Details

Structure
Ig superfamily, 44 kD
Distribution

All α/βTCR+ T cells, most γ/δTCR+ T cells, NK cell subset

Function
T cell costimulation
Ligand/Receptor
CD80 (B7-1) and CD86 (B7-2)
Cell Type
T cells, NK cells
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Tacke M, et al. 1995. J. Immunol. 154:5121.
2. Mitnancht R, et al. 1995. Eur. J. Immunol. 25:328.
3. Tacke M, et al. 1997. Eur. J. Immunol. 27:239.

Gene ID
25660 View all products for this Gene ID
UniProt
View information about CD28 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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