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- Clone
- OX-49 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Pgp-1, H-CAM, CD44s
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
LOU rat splenocytes stained with purified OX-49 conjugated to FITC
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 203901 | 25 µg | £45 | ||||
CD44 is an 80-95 kD cell surface glycoprotein. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. It is up-regulated when T cells and B cells are activated. It was reported that CD44 is a valuable marker for memory T cells. CD44 is an adhesion molecule involved in leukocyte adhesion and homing to lymphoid organs. The OX-49 antibody reacts with CD44H (known as CD44s) expressed on most leukocytes, except for a subset of B lymphocytes. The epitope recognized by OX-49 antibody has been mapped to a region on both the standard, CD44s, and the splice variant, CD44v, isoforms of CD44. However it was reported that OX-49 antibody cannot detect the CD44V isoform, possibly due to conformational changes in the epitope.
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Rat T blasts from mixed lymphocyte reactions
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC, IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Paterson DJ, et al. 1987. Mol Immunol. 24(12):1281
- Product Citations
-
- RRID
-
AB_1659203 (BioLegend Cat. No. 203901)
Antigen Details
- Structure
- A 80-95 kD cell surface glycoprotein
- Distribution
-
Expressed on most leukocytes. It is up-regulated when T cells and B cells are activated.
- Function
- Involve in leukocyte adhesion and homing to lymphoid organs.
- Ligand/Receptor
- Hyaluronate
- Cell Type
- B cells, Leukocytes, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Arch R, et al. 1992. Science 257 (5070):682
2. Mitnacht R, et al. 1995. Eur. J. Immunol. 25(2):328
3. Zheng Z, et al. 1995. J Cell Bio. 130(2):485
4. Westermann J, et al. 1994. Eur. J. Immunol. 24(10):2312 - Gene ID
- 12505 View all products for this Gene ID
- UniProt
- View information about CD44H on UniProt.org
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